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Core Bioreagents
Agarose Selection Guide | ||
Specialty Agarose Selection Guide
| General Agarose Selection Guide | |||||||
|---|---|---|---|---|---|---|---|
| Product Number | Application | Gel point °C |
Gel strength g/cm2 | Melting temperature °C | EEO | Sulfate % | Comments |
| Molecular Biology | |||||||
| A9539 | Routine nucleic acid separation | 36±1.5 | ≥1200 | 0.09-0.13 | ≤0.15 | For routine nucleic acid analysis by electrophoresis or Southern or Northern blotting. | |
| A5093 | Routine nucleic acid separation | 36±1.5 | ≥1200 | 0.09-0.13 | ≤0.15 | Low background when staiing with SYBR Green or ethidium bromide | |
| A9414 | Low melting point | 26-30 | ≥200 | ≤65 | ≤0.10 | ≤0.10 | For DNA recovery from low melting gels |
| A4718 | High resolution | ≤35 | ≥300 | ≤75 | ≤0.05 | For separation of small DNA fragments that differ in size by 2%. | |
| A7431 | Wide range/standard 3:1 | 32.5-38 | ≥1400 | ≤90 | ≤0.13 | ≤0.15 | Separates DNA fragments 10-1500 bp; gels less fragile compared to A 2790 |
| A2790 | Wide range | ≤35 | ≥500 | ≤65 | ≤0.15 | ≤0.15 | Separate low bp DNA, 50-1000 |
| A2929 | Pulse field running gel | 42±1 | ≥2400 | 0.04-0.07 | ≤0.08 | Separation of large DNA (10-2000 KB) by pulsed-field electrophoresis | |
| A3054 | Pulse field sample gel | ≤30 | ≥400 | ≤70 | ≤0.10 | ≤0.15 | For preparation of chromosomal DNA samples for pulsed-field electrophoresis |
| Protein Electrophoresis | |||||||
| A4679 | Immunoelctrophoresis | 36±1.5 | ≥1200 | 88 | 0.09-0.13 | For immunodiffusion and immunoelectrophoresis | |
| A9311 | Protein electrophoresis | 36±1.5 | ≥1000 | 80 | 0.16-0.19 | ≤0.20 | |
| A5304 | Protein electrophoresis | 36±1.5 | ≥700 | ≤90 | 0.3 | ≤0.30 | |
| Cell Culture | |||||||
| A9045 | General cell culture and insect cell culture | <30 | ≥200 | >60 | ≤0.1 | ≤0.10 | Clear gels, useful for media with heat labile components |
| A6560 | Plant cell culture | <30 | ≥200 | >60 | ≤0.1 | ≤0.10 | Clear gels, useful for media with heat labile components |
| Specialty Agarose Selection Guide | ||||||||
|---|---|---|---|---|---|---|---|---|
| Product Number | Type | Sulfate (%) | Gel Strength | Gel Point | Melting Temp. (°C) | Electroendosmosis (EEO) | ||
| g/cm2 | at % | °C | at % | |||||
| A0169 | I-A | <0.20 | >1200 >2500 |
1 1.5 |
36 ± 1.5 | 1.5 | 87 ± 1.5 | 0.09-0.13 |
| A0576 | I-B | ≤0.12 | ≥1800 ≥3200 |
1 1.5 |
36 ± 1.5 | 1.5 | 86 ± 2.0 | ≤0.12 |
| A6013 | I | ≤0.15 | ≥1200 | 1 | 36 ± 1.5 | 1.5 | 0.09-0.13 | |
| A9918 | II-A | <0.25 | >1000 >2000 |
1 1.5 |
36 ± 1.5 | 1.5 | 87 ± 1.5 | 0.16-0.19 |
| A6877 | II | ≤0.20 | ≥1000 | 1 | 36 ± 1.5 | 1.5 | 0.16-0.19 | |
| A9793 | III-A | <0.25 | >750 >1000 |
1 1.5 |
36 ± 1.5 | 1.5 | 87 ± 1.5 | 0.23-0.26 |
| A6138 | III | ≤0.20 | ≥650 | 1 | 36 ± 1.5 | 1.5 | 0.23-0.26 | |
| A3643 | IV | ≤0.25 | ≥650 | 1 | 36 ± 1.5 | 1.5 | ≥0.30 | |
| A3768 | V | ≤0.30 | ≥800 | 1 | 42 ± 1.5 | 1.5 | 0.10 | |
| A7174 | VI-A | ≤0.20 | >900 >1200 |
1 1.5 |
41 ± 1.5 | 1.5 | 95 ± 1.5 | ≤0.14 |
| A3893 | VI | ≤0.20 | ≥1000 | 1.5 | 42 ± 1.5 | 1.5 | 0.1 | |
| A0701 | VII-A | ≤0.10 | ≥250 ≥500 |
1 1.5 |
26 ± 2 | 1.5 | ≤65.5 | ≤0.12 |
| A4018 | VII | ≤0.10 | ≥200 | 1 | 26-30 | 1.5 | ≤65 | ≤0.1 |
| A4905 | VIII | ≤0.20 | ≥500 | 1 | ND | ±0.02 | ||
| A2576 | IX-A | ≤0.14 | ≥100 ≥400 |
1 1.5 |
≤17 | 1.5 | ≤60 | ≤0.11 |
| A5030 | IX | ≤0.10 | ≥75 | 2 | 8-17 | 0.8 | ≤50 | ≤0.05 |
| A3038 | XI | ≤0.15 | ≥500 | 4 | ≤35 | 4 | ≤65 | ≤0.15 |
| A7299 | XII | ≤0.20 | ≤500 ≤900 |
1 1.5 |
41 ± 1.5 | 1.5 | 95 ± 1.5 | ≤0.14 |