Antibiotic Selection Guide

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Antibiotics for Mammalian Cell Culture
Product Name	Product Number	Gram (+)bacteria	Gram (-)bacteria	Yeasts	Molds	Mycoplasma	Suggested Working Conc.
Amphotericin B	A2411			†	†		2.5 mg/L
Amphotericin B-Solubilized (Approx. 45%)	A9528			†	†		5.6 mg/L (of solid)
Amphotericin B(250 mg/ml solution)	A2942			†	†		10 ml/L
Ampicillin	A0166	†	†				100 mg/L
Antibiotic Antimycotic Solution (100x)(10,000 units penicillin, 10 mg streptomycin, and 25 µg amphotericin B per ml)	A5955	†	†	†	†		10 ml/L
Cephalothin	C3050	†	†				100 mg/L
Dihydrostreptomycin	D5155	†	†				100 mg/L
Erythromycin	E5389	†	†				100 mg/L
Gentamicin Sulfate	G1264	†	†			†	50 mg/L
Gentamicin Sulfate (10 mg/ml solution)	G1272	†	†			†	5 ml/L
Gentamicin Sulfate (50 mg/ml solution)	G1397	†	†			†	1 ml/L
L-Glutamine-Penicillin-Streptomycin Solution (200 mM L- glutamine, 10,000 units penicillin, and 10 mg streptomycinper ml)	G1146 G6784	†	†				10 ml/L
Kanamycin Monosulfate	K1377	†	†			†	100 ml/L
Kanamycin Sulfate (10 mg/ml solution)	K0129	†	†			†	10 ml/L
Kanamycin Sulfate (50 mg/ml solution)	K0254	†	†			†	2 ml/L
Lincomycin HCI	L2774	†					100 mg/L
Neomycin Sulfate	N6386	†	†				50 mg/L
Neomycin Sulfate (10 mg/ml solution)	N1142	†	†				5 ml/L
Nystatin (5,000 units nystatin per mg)	N6261			†	†		2.5 X 10⁵U/L (50 mg/L)
Nystatin	N4014			†	†		50 mg/L
Nystatin Suspension	N1638			†	†		24 ml/L
Paromomycin Sulfate	P5057	†	†				100mg mg/L
Penicillin-G (potassium salt)	P7794	†					100,00 U/L
Penicillin-G (sodium salt)	P3032	†					100,000 U/L
Penicillin-Streptomycin solution (5,000 units penicillin-G and 5 mg streptomycin per ml)	P4458	†	†				20 ml/L
Penicillin-Streptomycin Solution (10,000 units penicillin-G and 10 mg streptomycin per ml)	P0781 P7539 P4333	†	†				10 mg/mL
Penicillin-Streptomycin-Neomycin solution (5,000 units penicillin-G,5 mg streptomycin and 10 mg neomycin per ml)	P4083	†	†				10 ml/L
Phenoxymethylpenicillinic Acid (potassium salt) [Penicillin V]	P4807	†					100,000 U/L
Polymyxin B Sulfate	P4932		†				50 mg/L
SpectinomycinDihydrochloride	S4014	†	†				7.5-20 mg/L
Spectromycin Sulfate	S0890	†	†				10 mg/L
TetracyclineHydrochloride	T7660	†	†				10 mg/L
Tylosin Tartrate(8 mg/ml soln.)	T3397	†				†	1 ml/L

Antibiotics for Plant Cell Culture

Antibiotics
Product Name	Product Number	Gram (+)bacteria	Gram (-)bacteria	Mycobacteria	Mycoplasma	Suggested Working Conc.
Carbenicillin	C3416	‡	†			10-500
Cefotaxime	C7039	‡	†			up to 500 (CC, PR, EC)
Chloramphenicol	C1919	†	†	‡	‡	10-35 (TS)
G 418	G1279					up to 250 (CC)
Gentamicin Sulfate	G6896	‡	†		†	up to 500 (CC, PR, EC)
Hygormycin B	H9773					up to 500 (CC, PR, EC)
Kanamycin	K4378	†	†		†	16 (SSC); up to 40 (CC, PR, EC)
Paromycin	P8692	†	‡
Penicillin G, Potassium	P8306	†	‡			up to 100
Penicillin G, Sodium	P8431	†	‡			up to 100
Rifampicin	R7382	†	†	†		50 (TS); up to 25 (PC, TEC)
Streptomycin Sulfate	S0774	‡	†	‡		>16
Vancomycin	V1130	†				up to 10 (PC); up to 40 (CC, EC, STM)

Antimycotics
Product Name	Product Number	Yeasts	Molds	Suggested Working Conc.
Nystatin	N9767	†	†	16 (SSC); up to 40 (CC, EC, STM
Pentachloronitrobenzene	P8556		†
Thiabendazole	T5535		†

† = Effective against most species
‡ = Effective against certain species

¹Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant
selection; SE = somatic embryogenesis; SSC = stem selection culture; PC = protoplast culture; TEC = tuber explant culture;
STM = shoot tip micropropagation

Antibiotic Selection Agents for Cell Culture
Product Name	Product Number	Mode of Action	Suggested Working Conc.
Actinomycin D	A9415	Complexes with DNA and interferes with RNA synthesis	1 µg/ml
Bleomycin Sulfate	B8416	Complexes with DNA, causing strand scissions	10-100 µg/ml
Chloramphenicol	C3175	Inhibits elongation at peptidyl transferase	5 µg/ml
G 418	A1720	Blocks polypeptide synthesis and inhibits chain elongation	100-800 µg/ml
G 418 (50 mg/ml solution)	G8168	Blocks polypeptide synthesis and inhibits chain elongation	1 µg/ml
Hygromycin B	M4287	Blocks polypeptide synthesis and inhibits chain elongation
Mitomycin C	M4287	Inhibits nucleic acid synthesis	10-50 µg/ml
Mycophenolic Acid	M3536	Blocks inosine monophosphate dehydrogenase in guanosine monophosphate pathway	25 µg/ml
Puromycin HCl	P8833	Inhibits protein synthesis	10-100 µg/ml

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