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Antibiotic Selection Guide

 Antibiotics for Mammalian Cell Culture

 
Product Name Product No. Gram (+)bacteria Gram (-)bacteria Yeasts Molds Mycoplasma Suggested Working Conc.
Amphotericin B A2411       2.5 mg/L
Amphotericin B-Solubilized (Approx. 45%) A9528       5.6 mg/L (of solid)
Amphotericin B(250 mg/ml solution) A2942       10 ml/L
Ampicillin A0166       100 mg/L
Antibiotic Antimycotic Solution (100x)(10,000 units penicillin, 10 mg streptomycin, and 25 µg amphotericin B per ml) A5955   10 ml/L
Cephalothin C3050       100 mg/L
Dihydrostreptomycin D5155       100 mg/L
Erythromycin E5389       100 mg/L
Gentamicin Sulfate G1264     50 mg/L
Gentamicin Sulfate (10 mg/ml solution) G1272     5 ml/L
Gentamicin Sulfate (50 mg/ml solution) G1397     1 ml/L
L-Glutamine-Penicillin-Streptomycin Solution (200 mM L- glutamine, 10,000 units penicillin, and 10 mg streptomycinper ml) G1146 
G6784
      10 ml/L
Kanamycin Monosulfate K1377     100 ml/L
Kanamycin Sulfate (10 mg/ml solution) K0129     10 ml/L
Kanamycin Sulfate (50 mg/ml solution) K0254     2 ml/L
Lincomycin HCI L2774         100 mg/L
Neomycin Sulfate N6386       50 mg/L
Neomycin Sulfate (10 mg/ml solution) N1142       5 ml/L
Nystatin (5,000 units nystatin per mg) N6261       2.5 X 105U/L (50 mg/L)
Nystatin N4014       50 mg/L
Nystatin Suspension N1638       24 ml/L
Paromomycin Sulfate P5057       100mg mg/L
Penicillin-G (potassium salt) P7794         100,00 U/L
Penicillin-G (sodium salt) P3032         100,000 U/L
Penicillin-Streptomycin solution (5,000 units penicillin-G and 5 mg streptomycin per ml) P4458       20 ml/L
Penicillin-Streptomycin Solution (10,000 units penicillin-G and 10 mg streptomycin per ml) P0781 
P7539 
P4333
      10 mg/L
Penicillin-Streptomycin-Neomycin solution (5,000 units penicillin-G,5 mg streptomycin and 10 mg neomycin per ml) P4083       10 ml/L
Phenoxymethylpenicillinic Acid (potassium salt) [Penicillin V] P4807         100,000 U/L
Polymyxin B Sulfate P4932         50 mg/L
SpectinomycinDihydrochloride S4014       7.5-20 mg/L
Spectromycin Sulfate S0890       10 mg/L
TetracyclineHydrochloride T7660       10 mg/L
Tylosin Tartrate(8 mg/ml soln.) T3397       1 ml/L

 

 Antibiotics for Plant Cell Culture

Antibiotics

Product Name Product No. Gram (+)bacteria Gram (-)bacteria Mycobacteria Yeasts Molds Mycoplasma Suggested Working Conc.
Carbenicillin C3416         10-500
Cefotaxime C7039         up to 500 (CC, PR, EC)
Chloramphenicol C1919     10-35 (TS)
G 418 G1279             up to 250 (CC)
Gentamicin Sulfate G6896       up to 500 (CC, PR, EC)
Hygormycin B H9773             up to 500 (CC, PR, EC)
Kanamycin K4378       16 (SSC); up to 40 (CC, PR, EC)
Paromycin P8692          
Penicillin G, Potassium P8306         up to 100
Penicillin G, Sodium P8431         up to 100
Rifampicin R7382       50 (TS); up to 25 (PC, TEC)
Streptomycin Sulfate S0774       >16
Vancomycin V1130           up to 10 (PC); up to 40 (CC, EC, STM)

Antimycotics

Product Name Product No. Gram (+)bacteria Gram (-)bacteria Mycobacteria Yeasts Molds Mycoplasma Suggested Working Conc.
Nystatin N9767         16 (SSC); up to 40 (CC, EC, STM
Pentachloronitrobenzene P8556            
Thiabendazole T5535            

† = Effective against most species
‡ = Effective against certain species

1Application Key: CC = Callus Culture; PR = plant regeneration; EC = cotyledon, hypocotyl, or leaf disc culture; TS = transformant
selection; SE = somatic embryogenesis; SSC = stem selection culture; PC = protoplast culture; TEC = tuber explant culture;
STM = shoot tip micropropagation

 

 Antibiotic Selection Agents for Cell Culture

 
Product Name Product No. Mode of Action Suggested Working Conc.
Actinomycin D A9415 Complexes with DNA and interferes with RNA synthesis 1 µg/ml
Bleomycin Sulfate B8416 Complexes with DNA, causing strand scissions 10-100 µg/ml
Chloramphenicol C3175 Inhibits elongation at peptidyl transferase 5 µg/ml
G 418 A1720 Blocks polypeptide synthesis and inhibits chain elongation 100-800 µg/ml
G 418 (50 mg/ml solution) G8168 Blocks polypeptide synthesis and inhibits chain elongation 1 µg/ml
Hygromycin B M4287 Blocks polypeptide synthesis and inhibits chain elongation  
Mitomycin C M4287 Inhibits nucleic acid synthesis 10-50 µg/ml
Mycophenolic Acid M3536 Blocks inosine monophosphate dehydrogenase in guanosine monophosphate pathway 25 µg/ml
Puromycin HCl P8833 Inhibits protein synthesis 10-100 µg/ml