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Custom DNA Oligos
WellRED Primers
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Attention: The following specifications apply to the USA and Europe. All other countries, click here.
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For use on Beckman Coulter's CEQ/GeXP Genetic Analysis Systems.
- High-quality oligos
- Reliable service
- Competitively priced
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Sigma-Genosys is licensed by Beckman Coulter, Inc., to supply WellRED dye-labeled oligos designed for use with the CEQ/GeXP Genetic Analysis Systems and may be used for direct hybridization or in PCR amplification. WellRED dye-labeled oligos are DNA oligonucleotides labeled with WellRED dyes, D2, D3 or D4, at the 5'end. WellRed dye-labeled oligo specifications:
- Deprotected and desalted
- Purified by PAGE, RPC or RP-HPLC
- Available in lengths of 7 to 100 mers
- Delivered dried in individual, opaque tubes
- Shipped within 4 to 5 working days of receiving your order, pending successful QC validation
- Systematic QC by PAGE or mass spectrometry analysis
- Competitively priced
Benefits of WellRED Dye Chemistry Used with the CEQ/GeXP Genetic Analysis Systems
1. WellRED oligos use cyanine-based fluorescent dyes with high extinction coefficients that absorb in the near-infrared region, thereby reducing background noise from biological materials, resulting in highly sensitive detection.
2. The CEQ/GeXP Genetic Analysis Systems enable DNA samples to be kept in linear form during analysis. The CEQ/GeXP Genetic Analysis Systems' online denaturation process prevents any reformation of secondary structures, especially during a long run without any manual additional step.
3. WellRED dye chemistry and pre-heating enable the CEQ/GeXP Genetic Analysis Systems to resolve complex structures, where other systems fail.
Guaranteed Yields and Estimated Assays for WellRED Dye-labeled Oligos
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PAGE |
RPC |
RP-HPLC |
| Guaranteed yield (OD) |
1 |
2 |
4 |
5 |
10 |
50 |
100 |
| Approx. yield (nmols*) |
5 |
10 |
20 |
25 |
50 |
250 |
500 |
| Estimated assays (reactions) |
500
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1,000 |
1,000
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2,000 |
2,000
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4,000 |
2,500
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5,000 |
5,000
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10,000 |
25,000
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50,000 |
50,000
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100,000 |
Spectral Properties of WellRED Dyes
| WellRED oligo |
Excitation maximum (nm) |
Emission maximum (nm) |
Molar extinction coefficient |
| D2-PA |
750 |
770 |
170,000 |
| D3-PA |
685 |
706 |
224,000 |
| D4-PA |
650 |
670 |
203,000 |
*CEQ/GeXP is a trademark of Beckman Coulter, Inc.
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