Custom Formats for High-Throughput Applications

Whether your application requires a few oligos or greater than 100,000 oligos, Sigma^® will format your oligos to best suit your application as well as your automation and information requirements.

Custom specifications can include:

• 96 or 384 well plates: Matrix tubes, Micronic tubes, deep & shallow well plates
• Concentration normalization options with fixed or variable volumes
• Dried or in solution (water or TE)

In addition to the common formats listed above, other options are available including:

• 1-D or 2-D Barcoding
• Customer Specified Plate and Buffers 
• Daughter Plates
• Forward & Reverse Primers in a Single Well

Quality
All oligonucleotides are verified by mass spectrometry. Each shipment includes data sheets for import into customers’ information systems containing well location, sequence, oligo name, lot number, MW, μg/OD, length, Tm, concentration and volume (in solution).

Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:

• Plate orientation and well position verification by mass spectroscopy
• Mixed Primers, i.e. Forward and Reverse in a single well, verified by mass spectroscopy , see example below
• Verification for machine readable barcodes (1D and 2D)
• Visual inspection of all master and daughter plates prior to shipment

Concentration Verification and Plate Identity
Sigma employs a variety of liquid-handling automation systems to normalize the concentration of the oligonucleotides. A multi-step normalization process ensures the concentrations are within a narrow range (±10%). At the end of the process, a final absorbance reading (measured at 260nm) ensures that every oligo is at the desired concentration.

Delivery
Standard delivery is 3–5 working days. Large projects will be placed on a delivery schedule based upon your needs.

Ordering
Orders may be placed by our secure website or by E-mail. Contact your local office for details.

Pricing and Quoting
Please contact your local Sigma-Aldrich office to discuss your project and request a quotation.

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