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Custom DNA Oligos
RNA Oligonucleotide Synthesis (7-45 mers)
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Attention: The following specifications apply to the USA and Europe.
All other countries, click here. |
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Product Line:
| Quality |
Desalted |
| Synthesis Scale |
0.05 µmole |
0.2 µmole |
1.0 µmole |
| Standard RNA |
X |
X |
X |
| Mixed RNA/DNA/2-OMe/LNA® |
X |
X |
X |
| Phosphorothioate linkages (s-oligos) |
X |
X |
X |
Guaranteed Yields (7-45 mers):
| Synthesis Scale |
0.05 µmole |
0.2 µmole |
1.0 µmole |
| Desalted |
5 OD (150µg) |
12 OD (360µg) |
40 OD (1200µg) |
| RP-HPLC |
1 OD (30µg) |
2.5 OD (75µg) |
13 OD (390µg) |
| PAGE |
0.5 OD (15µg) |
1 OD (30µg) |
5 OD (150µg) |
Purification:
| Quality |
Purified |
| Synthesis Scale |
0.05 µmole |
0.2 µmole |
1.0 µmole |
| RP-HPLC |
X |
X |
X |
| PAGE |
X |
X |
X |
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Quality
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• fully deprotected and desalted, purified by PAGE or RP-HPLC on request
• it is highly recommended to use purified RNA for oligos > 30 mers.
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Form
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• RNA and 2' O-Methyl mixed oligos are sent dry
• Quantity (OD and nmoles) and concentration (µM and µg/µl)
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Quality Controls
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See information on our oligos: strict quality controls
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Guarantee
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Guaranteed sequence, quality and quantity
Please see yield table above for guaranteed minimum yields
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Documentation
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All Oligos are delivered with an Oligonucleotide Technical Data Sheet that can also be sent through e-mail
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Additional Services
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• Aliquoting
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Synthesis Time
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• 6 days for standard RNA
• 7 days for standard or PAGE purified mixed oligos
• 10 days for RP-HPLC purified oligos
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Storage
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Although oligonucleotides are stable at 4°C for up to 2 weeks, Sigma recommends storing at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, RNA oligos should be dried.
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LNA (Locked Nucleic Acids) is a registered trademark of Exiqon A/S.
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