| Synthesis Scales | 0.025, 0.05, 0.2, 1.0, 10, and 15µmole mg - gram quantity manufacturing available upon request |
| Purification | Desalt, Cartridge, HPLC, PAGE See Oligonucleotide Purification Guidelines |
| Length | 10 – 120 bases |
| Backbone | Phosphodiester / Phosphorothioate |
| Format | Dry or In Solution (normalized) |
| Modifications | Over 200 available, including dyes (fluorescent and non-fluorescent) |
| Packaging | 2 mL - 50 mL Tubes, Microtiter Plates |
| Quality Controls | MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy |
| Technical Datasheet & Labels |
Includes yield, Tm (melting temperature), MW, and µg/OD Duplicate set of labels with each order |
| Additional Services | Concentration adjustment Aliquoting into tubes or well plates MSQC traces available upon request |
Sigma can format your oligos to best suit your application as well as your automation and information requirements.
Custom specifications can include:
In addition to the common formats listed above, other options are available including:
Quality
Each shipment includes data sheets for import into customers’ information systems containing well location, sequence, oligo name, lot number, MW, µg/OD, length, Tm, concentration and volume (in solution).
Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:
Learn more about our quality control and quality assurance.
Concentration Verification and Plate Identity
Sigma employs a variety of liquid-handling automation systems to normalize the oligo concentration. A multi-step normalization process ensures the concentrations are within a narrow range. At the end of the process, a final absorbance reading (measured at 260nm) ensures that every oligo is at the desired concentration.
| Scale (µmole) | Desalt | Cartridge | HPLC | PAGE |
|---|---|---|---|---|
| 0.025 | 24 hours | NA | NA | NA |
| 0.05 | 1-2 days | 1-2 days | 3-4 days | 3-4 days |
| 0.2 | 1-2 days | 1-2 days | 3-4 days | 3-4 days |
| 1.0 | 1-2 days | 1-2 days | 3-4 days | 3-4 days |
| Plates (non-modified)* | 3-5 days | 3-5 days | Inquire | Inquire |
| Greater than 1.0 µmole | 10 days or less | 10 days or less | 10 days or less | 10 days or less |
| Large quantity | Inquire | Inquire | Inquire | Inquire |
| Post-synthesis modified | 7-10 days | 7-10 days | 7-10 days | 7-10 days |
| All other modifications | 5-7 days or less | 5-7 days or less | 5-7 days or less | 5-7 days or less |
* Large projects will be placed on a delivery schedule based upon the customers project needs.
| Non-Modified Oligos (O.D./µg)* | ||||
|---|---|---|---|---|
| Scale (µmole) | Desalt | Cartridge | HPLC | PAGE |
| 0.025 | 3/90 | NA | NA | NA |
| 0.05 | 5/150 | 1/30 | 1/30 | 0.5/15 |
| 0.2 | 12/360 | 3/90 | 2.5/75 | 1/30 |
| 1.0 | 40/1,200 | 12/360 | 13/390 | 5/150 |
| 10 | 400/12,000 | NA | 130/3,900 | NA |
| 15 | 600/18,000 | NA | 190/5,700 | NA |
* Guarantee is for 20 mers or longer. Shorter oligos may have fewer ODs.