|Synthesis Scales||0.025, 0.05, 0.2, 1.0, 10, and 15µmole
mg - gram quantity manufacturing available upon request
|Purification||Desalt, Cartridge, HPLC, PAGE
See Oligonucleotide Purification Guidelines
|Length||10 – 120 bases|
|Backbone||Phosphodiester / Phosphorothioate|
|Format||Dry or In Solution (normalized)|
|Modifications||Over 200 available, including dyes (fluorescent and non-fluorescent)|
|Packaging||2 mL - 50 mL Tubes, Microtiter Plates|
|Quality Controls||MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy|
|Includes yield, Tm (melting temperature), MW, and µg/OD
Duplicate set of labels with each order
|Additional Services||Concentration adjustment
Aliquoting into tubes or well plates
MSQC traces available upon request
Sigma can format your oligos to best suit your application as well as your automation and information requirements.
Custom specifications can include:
In addition to the common formats listed above, other options are available including:
Each shipment includes data sheets for import into customers’ information systems containing well location, sequence, oligo name, lot number, MW, µg/OD, length, Tm, concentration and volume (in solution).
Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:
Learn more about our quality control and quality assurance.
Concentration Verification and Plate Identity
Sigma employs a variety of liquid-handling automation systems to normalize the oligo concentration. A multi-step normalization process ensures the concentrations are within a narrow range. At the end of the process, a final absorbance reading (measured at 260nm) ensures that every oligo is at the desired concentration.
|0.05||1-2 days||1-2 days||3-4 days||3-4 days|
|0.2||1-2 days||1-2 days||3-4 days||3-4 days|
|1.0||1-2 days||1-2 days||3-4 days||3-4 days|
|Plates (non-modified)*||3-5 days||3-5 days||Inquire||Inquire|
|Greater than 1.0 µmole||10 days or less||10 days or less||10 days or less||10 days or less|
|Post-synthesis modified||7-10 days||7-10 days||7-10 days||7-10 days|
|All other modifications||5-7 days or less||5-7 days or less||5-7 days or less||5-7 days or less|
* Large projects will be placed on a delivery schedule based upon the customers project needs.
|Non-Modified Oligos (O.D./µg)*|
* Guarantee is for 20 mers or longer. Shorter oligos may have fewer ODs.