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Custom Peptides

PEPscreen® Calculator Tables

Welcome to the Peptide Library Design & Calculator tool presented by Sigma-Aldrich You may launch the Design Tool and reference these tables at the same time, or view the full instructions for getting started.

The protein sequence of interest should use the following letter designations.


Amino Acid
3 letter code
1 letter code
Alanine
Ala
A
Cysteine
Cys
C
Aspartic acid
Asp
D
Glutamic acid
Glu
E
Phenylalanine
Phe
F
Glycine
Gly
G
Histidine
His
H
Isoleucine
Ile
I
Lysine
Lys
K
Leucine
Leu
L
Methionine
Met
M
Asparagine
Asn
N
Proline
Pro
P
Glutamine
Gln
Q
Arginine
Arg
R
Serine
Ser
S
Threonine
Thr
T
Valine
Val
V
Tryptophan
Trp
W
Tyrosine
Tyr
Y


Appropriate peptide length and amino acid overlap:

Potential effects of the different combinations between peptide length and offset number

relationship length offset



Hydropathy values presented are the weighted average based upon known hydropathy values per amino acid divided by the length of the peptide sequence. The values are based on model systems of free energy transfer of acetyl-amino acyl amides (Ac-AA-am) by Fauchere & Pliska and based on the RP-HPLC retention times of peptide model systems at pH 7 by Monera.


Weighted average Peptide hydrophobicity classification Solubility
1 to 2.25 Very hydrophobic Very difficult to dissolve
0.6 to 0.9 Moderately hydrophobic Difficult to dissolve
0.1 to 0.5 Hydrophobic Likely soluble
-0.6 to 0 Hydrophilic Soluble
-1 to –0.5 Very hydrophilic Very soluble


Correlations between Fauchere & Pliska and Monera et al Hydrophobicity Scales are shown below:

Hydrophobicity Scales



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