PEPscreen^® Peptides

Sigma^® has developed a proprietary custom peptide synthesis platform utilizing Fmoc chemistry, providing a solution to enhance peptide screening applications. Large numbers of peptides, in 96-well arrays, are delivered quickly and affordably. MALDI-TOF mass spec is performed on 100% of the samples.

PEPscreen Library Design Tool

PEPscreen Brochure Online

Product Specifications:

• Low cost per peptide
• Amount: 0.5 - 2 mg or 2 - 5 mg
• Length: 2 - 20 amino acids
• Comprehensive Modification Offering
• MALDI-ToF Mass Spectrometry on 100% of peptides
• Peptides supplied dried and in 96-well tube rack format
• Dispatch: approximately 7 business days
• Minimum order size is 24 peptides

Internal Testing of Synthesis Platform:

• We routinely run tests of this platform to ensure the quality of the product.
  
• Average Crude Purity of 10mers: ~86%
  
• Average Crude Purity of 15mers: ~73%
  
• Average Crude Purity of 20mers: ~61%
  
• Purity of ACP peptide at different positions on a 96-well plate: ~92% +/- 2%

Technical Resources:

• Epitope Mapping and Vaccine Research: Vaccination as a Strategic Medical Option
• Frequently Asked Questions for PEPscreen Peptides
• Generalized Steps of the ELISPOT Assay Procedure
• Guidelines for Dissolving PEPscreen Peptide Sets (253 Kb PDF)
• Storage and Handling Guidelines (689 Kb PDF)

Application Data Now Available!
The following application data was received from customers using our PEPscreen Technology:

• Diverse CD4 Response to Vaccinia Virus in Human is Revealed by Proteome-Wide T cell Profiling
• Binding and Kinetic Analysis of Protein Interactions Probed by Label-Free SpotMatrix SPR Technology in a Peptide Chip Format
• Cross-CladeCD8+ T-Cell Responses with a Preference for the Predominant Circulating Clade
• Development of Peptide-based Microarrays for Screening Antibodies from Patients with Autoimmune Diseases
• High-throughput Screening of Peptide Substrates for Tyrosine Kinases Using PEPscreen Custom Peptide Library
• Use of Peptide Libraries to Investigate the Evolutionary Preservation of Antimicrobial Properties of Complement Peptide C3a from Invertebrates to Humans
• Use of PEPscreen Libraries to Identify CD8 Epitopes Presented During Malaria Infection
• Use of PEPscreen Peptides to Investigate CTL Class I MHC and Th Class II Responses to AAV-hF.IX Gene Therapy
• A Quantitative Mass Spectrometry-Based Approach for Identifying Protein Kinase Clients and Quantifying Kinase Activity
• Use of PEPscreen Peptides to Investigate Protein Farnesyltransferase Substrate Specificity

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