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Custom Oligos
Custom RNA Oligos
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Product Line
| Quality |
Desalted |
| Synthesis Scale |
0.05 µmole |
0.2 µmole |
1.0 µmole |
| Standard RNA |
X |
X |
X |
| Mixed RNA/DNA/2-OMe/LNA® |
X |
X |
X |
| Phosphorothioate linkages (s-oligos) |
X |
X |
X |
Guaranteed Yields (7-45 mers)
| Synthesis Scale |
0.05 µmole |
0.2 µmole |
1.0 µmole |
| Desalted |
5 OD (150µg) |
12 OD (360µg) |
40 OD (1200µg) |
| RP-HPLC |
1 OD (30µg) |
2.5 OD (75µg) |
13 OD (390µg) |
| PAGE |
0.5 OD (15µg) |
1 OD (30µg) |
5 OD (150µg) |
Purification
| Quality |
Purified |
| Synthesis Scale |
0.05 µmole |
0.2 µmole |
1.0 µmole |
| RP-HPLC |
X |
X |
X |
| PAGE |
X |
X |
X |
| Quality |
• fully deprotected and desalted, purified by PAGE or RP-HPLC on request
• it is highly recommended to use purified RNA for oligos > 30 mers. |
| Form |
• RNA and 2' O-Methyl mixed oligos are sent dry
• Quantity (OD and nmoles) and concentration (µM and µg/µl) |
| Quality Controls |
Every oligo is quality checked using one or more of the following techniques: MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), or polyacrylamide gel electrophoresis (PAGE). |
| Guarantee |
Guaranteed sequence, quality and quantity
Please see yield table above for guaranteed minimum yields |
| Documentation |
All oligos are delivered with a Quality Assurance document including yields, Tm (melting temperature), MW, and µg/OD. |
| Additional Services |
• Aliquoting |
| Synthesis Time |
• 6 days for standard RNA
• 7 days for standard or PAGE purified mixed oligos
• 10 days for RP-HPLC purified oligos |
| Storage |
Although oligonucleotides are stable at 4°C for up to 2 weeks, Sigma recommends storing at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, RNA oligos should be dried. |
LNA (Locked Nucleic Acids) is a registered trademark of Exiqon A/S.
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