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Custom Oligos
Custom RNA Oligos | ||
Product Line
| Quality | Desalted | |||||
| Synthesis Scale | 0.05 µmole | 0.2 µmole | 1.0 µmole | |||
| Standard RNA | X | X | X | |||
| Mixed RNA/DNA/2-OMe/LNA® | X | X | X | |||
| Phosphorothioate linkages (s-oligos) | X | X | X | |||
Guaranteed Yields (7-45 mers)
| Synthesis Scale | 0.05 µmole | 0.2 µmole | 1.0 µmole |
| Desalted | 5 OD (150µg) | 12 OD (360µg) | 40 OD (1200µg) |
| RP-HPLC | 1 OD (30µg) | 2.5 OD (75µg) | 13 OD (390µg) |
| PAGE | 0.5 OD (15µg) | 1 OD (30µg) | 5 OD (150µg) |
Purification
| Quality | Purified | ||||
| Synthesis Scale | 0.05 µmole | 0.2 µmole | 1.0 µmole | ||
| RP-HPLC | X | X | X | ||
| PAGE | X | X | X | ||
| Quality | • fully deprotected and desalted, purified by PAGE or RP-HPLC on request • it is highly recommended to use purified RNA for oligos > 30 mers. |
| Form | • RNA and 2' O-Methyl mixed oligos are sent dry • Quantity (OD and nmoles) and concentration (µM and µg/µl) |
| Quality Controls | Every oligo is quality checked using one or more of the following techniques: MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), or polyacrylamide gel electrophoresis (PAGE). |
| Guarantee | Guaranteed sequence, quality and quantity Please see yield table above for guaranteed minimum yields |
| Documentation | All oligos are delivered with a Quality Assurance document including yields, Tm (melting temperature), MW, and µg/OD. |
| Additional Services | • Aliquoting |
| Synthesis Time | • 6 days for standard RNA • 7 days for standard or PAGE purified mixed oligos • 10 days for RP-HPLC purified oligos |
| Storage | Although oligonucleotides are stable at 4°C for up to 2 weeks, Sigma recommends storing at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, RNA oligos should be dried. |
LNA (Locked Nucleic Acids) is a registered trademark of Exiqon A/S.