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Sigma–Genosys offers dual–labeled probes for use in applications such as real–time PCR, multiplexing, SNP analysis, allelic discrimination and spectral genotyping. We are a licensed supplier of Molecular Beacons and Black Hole Quenchers.
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Capabilities
Dual–labeled fluorogenic probes and Molecular Beacons incorporate a 5'–reporter dye and a 3'–quencher within the probe design. Sigma–Genosys can provide the traditional TAMRA quencher for dual-labeled fluorogenic probes and DABCYL quencher for Molecular Beacons.
However, a new family of quenchers is now available that has been optimized for FRET – the Black Hole Quencher™. Sigma–Genosys is licensed to sell BHQ™ labeled oligos for optimum quenching efficiency. Each BHQ has been designed to work with specific fluorophores. BHQ is suitable for both dual–labeled fluorogenic probes and Molecular Beacons.
| Quencher |
Max Abs |
Quenching Range |
Fluorophores |
| BHQ–1™ |
534 nm |
480 – 580 nm |
FAM, HEX, TET, JOE |
| BHQ–2™ |
579 nm |
550 – 650 nm |
TAMRA, ROX, Cy3™ |
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Expected Yields
Probes are available with yield guarantees from 1 OD – 100 OD. Package size options will vary by region. Contact your local office to confirm availability. Scale options for your region can also be viewed through your local online ordering system.
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Purification
In order to ensure that there is no background fluorescence, HPLC purification is standard with all dual–labeled probes.
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Guaranteed Quality
Oligos are routinely checked for length, purity, and composition. Our global quality control capabilities include MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and polyacrylamide gel electrophoresis (PAGE). In addition, the signal–to–background ratio is measured against a target sequence for all Molecular Beacons. If you are dissatisfied and report an oligo problem within 30 days of order receipt, we will gladly remake your oligo free of charge or provide a credit for the purchase amount.
All orders are shipped with a Quality Assurance document that includes measured yields, melting temperature, MW and µg/OD with every oligo. Oligos in tubes are accompanied by an extra vial label for your records.
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Shipping Schedule
Expected shipment time for most dual–labeled probes is 3-5 days.
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Pricing
Pricing for dual–labeled fluorogenic probes and Molecular Beacons includes the 5'–reporter dye, 3'–quencher, and HPLC purification. Base charges may apply. Contact your local sales office for details or to request a large volume quote.
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