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Life Science > Custom Oligos > Custom DNA Probes > Product Lines > Fluorescent Probes > LightCycler® Probes > Reporters Quenchers
Dual-Labeled DNA Probes

Reporters Quenchers

Spectral properties of fluorophores

The table below details the most commonly used dyes for the fluorescent probes, together with their excitation and emission data. Please enquire for any other labels.


Quenchers

Maximum absorbance (nm)

Maximum emission (nm)

BHQ-1 Dark Quencher

535

None

BHQ-2 Dark Quencher

579

None

DABCYL Dark Quencher

453

None

DABCYL-dT Dark Quencher

453

None

TAMRA

550

576

Labels

Maximum absorbance (nm)

Maximum emission (nm)

6-FAM

495

517

Cy3

550

570

Cy5

650

667

Cy5.5

675

694

Fluorescein

495

520

HEX

537

553

JOE

520

548

LightCycler Red 610

590

610

LightCycler Red 640

625

640

LightCycler Red 670

650

670

LightCycler Red 705

680

705

Oregon Green 488

495

521

Oregon Green 500

499

519

Oregon Green 514

506

526

Rhodamine

564

603

6-CarboxyRhodamine 6G

524

557

Rhodamine Green

504

532

Rhodamine Red

570

590

ROX

581

607

TAMRA

550

576

TET

521

538

Texas Red

589

610

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