Black Hole Quenchers

Some commonly used quenchers-such as DABCYL and TAMRA-suffer from a number of drawbacks, including poor spectral overlap between the fluorescent dye and quencher molecule (DABCYL) or inherent fluorescence of the quencher (TAMRA)-resulting in a relatively poor signal-to-noise ratio. Black Hole Quencher™ (BHQ™) molecules have been developed to overcome these drawbacks.

Benefits:

No native fluorescence, resulting in lower background fluorescence
Increases signal-to-noise ratios, providing higher sensitivity
Accesses the visible spectrum into near-IR for reporting (480 to 730 nm)
Maximizes spectral overlap, increasing efficiency of quenching
Enables wider choice of reporter dyes for multiplexing assays

Absorption Spectra of BHQ dyes among the most commonly used dyes.

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