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Dual-Labeled DNA Probes

LNA® Dual-Labeled Fluorogenic Probes and Molecular Beacons

For your convenience, LNA dual-labeled fluorogenic probes and LNA molecular beacons are available at a fixed price per oligonucleotide, within the following parameters:

  • Up to 8 LNA bases
  • Deprotected, desalted and purified by PAGE or RP-HPLC
  • Available in lengths of 7 to 40 mers
  • Delivered dried in individual, opaque tubes
  • Shipped in 7 working days of receiving your order, pending successful QC validation*

Ordering information


Guaranteed yields of LNA dual-labeled fluorogenic probes and
LNA molecular beacons
Guaranteed yield (OD)
Approximate yield (nmols**)
Estimated assays (reactions***)
1
5
500
3
15
1,500
5
25
2,500
10
50
10,000

** Estimate 1 OD = 5 nmols = 30 µg, for a 20 mer oligo
*** Estimated number of assays based on a 50 µl reaction volume.
Please enquire for alternative quantities.

Labels for LNA dual-labeled fluorogenic probes and
LNA molecular beacons
5'-end reporter
3'-end quencher
6-FAM™, HEX™ or TET™
TAMRA™, DABCYL, BHQ™-1
or BHQ-2

Please inquire about optional dye labels.


Figure 1: LNA dual-labeled fluorogenic probes vs. TaqMan® MGB probes in 5' nuclease PCR assays. (Amplification of Staphylococcal enterotoxin gene in a Staphylococcus aureus strain11).

PP_LNAFP_QPCR
Key
PP_LNAFP_Key
 LNA dual-labeled fluorogenic probe
PP_LNAFP_Key_Green
 TaqMan MGB probe

 


OD = optical density

* Please check with your Sigma Custom Products representative for local delivery times.

Specifications are subject to change.


Image of Beacon Designer: Software for Real Time PCR Oligo Design

LNA—Notice of limited rights: Sigma, Sigma-Life Science are only licensed to sell Dual Labeled Fluorescent Probes, LightCycler Probes, Scorpion Probes and Molecular Beacons containing LNA (Locked Nucleic Acids) for use in Real-Time PCR.
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