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Dual-Labeled DNA Probes
LNA LightCycler® Probes
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Attention: The following specifications apply to the USA and Europe. All other countries, click here.
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For your convenience, LNA LightCycler probes are available at a fixed price per oligonucleotide, within the following parameters:
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- Up to 6 LNA bases
- Deprotected, desalted and purified by RP-HPLC
- Available in lengths of 7 to 40 mers
- Delivered dried in individual, opaque tubes
- Shipped in 6 working days of receiving your order, pending successful QC validation*
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Guaranteed yields of LNA LightCycler probes
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Guaranteed yield (OD)
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Approximate yield (nmols)
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Estimated assays (reactions)
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0.1
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0.3
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100
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0.25
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1
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300
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1.5
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3
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1,000
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Please inquire for alternative quantities.
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Labels and modifications for LNA LightCycler probes
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LNA Probe I
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LNA Probe 2
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3'-end donor fluorophore
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3'-end
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5'-end acceptor fluorophore
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Labels and modifications
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LightCycler Fluorescein
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Phosphate
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LightCycler Red 640
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Note: Fluorescent signaling occurs during the annealing step of Q.PCR.
Please inquire for optional dye labels.
Figure 1: Melting curve profiles for Human Cystic Fibrosis SNP G542X demonstrate the superior discrimination power of LNA LightCycler probes over DNA LightCycler probes.Figure 1a: A set of DNA LightCycler probes (A-25; D-22)
Key
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Wild-type template |
| II |
Heterozygous template |
| III |
Mutant-type template |
| IV |
Control without template |
Figure 1b: A set of LNA LightCycler probes (A-16; D-16)
Key
| I |
Wild-type template |
| II |
Heterozygous template |
| III |
Mutant-type template |
Note that DNA LightCycler probes do not pick up on the presence of the homozygous mutant, whereas the LNA LightCycler probes are able to identify the presence of all three template types, including the homozygous mutant.
* Please check with your Sigma-Proligo representative for local delivery times.
Specifications are subject to change.
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