OligoArchitect™ Consultative

Request Design

For complex and demanding applications, utilize our consultative service to ensure the success of your PCR, qPCR, or other assays. With personal consultation from our expert, technical support scientists, your request, including all sequences and data analysis, will be MIQE compliant and usually returned to you within 24 hours. If required, you will also receive follow-up assay optimization, data analysis assistance, and troubleshooting support.

Specifications
Applications	Detection Chemistries	Modifications
Traditional PCR Methylation Analysis Endpoint Genotyping Gene Expression Analysis Genomic Copy Number Determination Transcript Detection Across Exon Junctions High Resolution Melting Analysis Allele Discrimination SNP Detection Haplotyping Multiplexing Northern Blotting Southern Blotting Fluorescence in situ Hybridization	SYBR^® Green I Primers Dual-Labeled Probes¹ LightCycler^® Probes Scorpions™ Probes Molecular Beacons	LNA^® within probes and beacons only

1 Also known as hydrolysis probes, which are used in the 5’ nuclease assay.

Sigma’s consultative service uses Beacon Designer™ from Premier Biosoft International. Blastn and mFold are used to determine assay specificity, a MIQE requirement.

If you need assistance, email: oligotechserv@sial.com.

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