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Custom Oligos
Custom siRNA Oligos
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Through the powerful science of RNA interference, successful gene silencing can be achieved with the use of synthetic, small interfering RNA (siRNA). By targeting these small, synthetic oligos, typically 19-23 bases in length against a specific gene of interest researchers are identifying gene function, elucidating pathways, and screening for potential new drug targets.
Features
- New: FAST siRNAs with ultra-fast turn-around time
- New: Modified nucleotides now available for siRNA products
- Deprotected, desalted and ready-to-use
- µg to multi-gram capacity
- Available in duplex or single-stranded form
- Purified by PAGE or RP-HPLC upon request
- Comprehensive QC and technical data sheet provided with each siRNA
- Rapid turn-around time and high throughput capacity
New siRNA Features
FAST siRNAs
Get your siRNAs faster than ever before. FAST siRNAs are produced in only 3 days. Available in 2 OD and 5 OD format.
Modified nucleotides available for custom siRNAs!
2'OMe bases have been used successfully to increase the serum stability of siRNA duplexes and are of particular interest for in vivo applications of siRNAs. Try modified siRNAs for your next in vivo experiment.
Unpurified siRNA is as effective as purified siRNA
For successful knockdown of your gene of interest, order our siRNA (small interfering RNA) oligos. Our unpurified siRNA oligos are as effective in reducing gene expression as purified siRNA oligos in cell culture experiments, making them very cost effective. All our siRNA oligos are deprotected, desalted and ready-to-use.
For siRNA usage in animal models please see our MISSION® In Vivo Quality and iScale Oligos™ siRNA website.
Superior Manufacturing Capacity
With a capacity to synthesize over one million siRNA oligos per year, Sigma offers reliable delivery times, no matter how many oligonucleotides you order.
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RNA Chemistry and Proprietary Technologies of Sigma
Our RNA synthesis relies on the use of fast deprotection ribonucleoside phosphoramidites protected at the 2' position by a tert-butyldimethylsilyl (TBDMS) group and, at the 3' position, by a phosphoramidite.
RNA synthesis and deprotection are performed using Sigma's proprietary technologies ie. Ultra Fast Parallel Synthesis (UFPS) and Ultra Fast Parallel Deprotection (UFPD). The use of these technologies means higher coupling efficiency and faster deprotection, resulting in higher quality synthesis and faster turn-around times.
Specifications of siRNA Oligos
| Quality control |
All our siRNA oligos undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE or mass spectrometry analysis. Quantity is systematically validated by UV absorbance at 260 nm. |
| Purification |
Fully deprotected and desalted
Purified by RP-HPLC upon request |
| Delivered Quantity |
2 OD (~10 nmol), 5 OD (~25 nmol), 10 OD (~50 nmol) |
| Length |
19 to 27 mers |
| Bases |
RNA
DNA
2'OMe |
| Backbone |
Phosphodiester bond |
| Labels and modifications |
Amine, biotin, Cy-dyes, fluorescein, phosphate. Other labels available upon request |
| Format |
Simplex siRNAs are delivered dry.
Duplex siRNA oligos are delivered dry or liquid |
| Storage and stability |
Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, Sigma recommends storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, siRNA oligos should be dried. Oligonucleotides with fluorescent labels should be protected from light. Sigma guarantees its oligonucleotides for six months, when stored under the above conditions. |
| Shipment |
Shipped by express delivery, dry or liquid in individual, transparent tubes |
Oligonucleotide
Technical Data
Sheet |
Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, size, extinction coefficient and purification data. Additional gel images are provided for duplex confirmation upon request. |
| Services available upon request |
Aliquoting
Free design support
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| Pricing |
Please contact your local sales representative |
| Ordering |
On-line, by email or by fax |
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Related Products
Pre-designed siRNA
MISSION siRNA Human Druggable Genome
MISSION siRNA Rat Druggable Genome
MISSION siRNA Mouse Kinase Library
siRNA Literature for Download
Sales Brochure (626 Kb PDF)
siRNA Users Guide by Tuschl et al.
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