Custom siRNA Oligos

On this page:

Features
Superior Manufacturing Capacity
RNA Chemistry and Proprietary Technologies of Sigma
Specifications of siRNA Oligos
siRNA Pricing

Through the powerful science of RNA interference, successful gene silencing can be achieved with the use of synthetic, small interfering RNA (siRNA). By targeting these small, synthetic oligos, typically 19-23 bases in length against a specific gene of interest researchers are identifying gene function, elucidating pathways, and screening for potential new drug targets.

Features

New: FAST siRNAs with ultra-fast turn-around time
New: Modified nucleotides now available for siRNA products
Deprotected, desalted and ready-to-use
µg to multi-gram capacity
Available in duplex or single-stranded form
Purified by PAGE or RP-HPLC upon request
Comprehensive QC and technical data sheet provided with each siRNA
Rapid turn-around time and high throughput capacity

New siRNA Features

FAST siRNAs
Get your siRNAs faster than ever before. FAST siRNAs are produced in only 3 days. Available in 2 OD and 5 OD format.

Modified nucleotides available for custom siRNAs!
2'OMe bases have been used successfully to increase the serum stability of siRNA duplexes and are of particular interest for in vivo applications of siRNAs. Try modified siRNAs for your next in vivo experiment.

Unpurified siRNA is as effective as purified siRNA

For successful knockdown of your gene of interest, order our siRNA (small interfering RNA) oligos. Our unpurified siRNA oligos are as effective in reducing gene expression as purified siRNA oligos in cell culture experiments, making them very cost effective. All our siRNA oligos are deprotected, desalted and ready-to-use.

For siRNA usage in animal models please see our MISSION^® In Vivo Quality and iScale Oligos™ siRNA website.

Superior Manufacturing Capacity

With a capacity to synthesize over one million siRNA oligos per year, Sigma offers reliable delivery times, no matter how many oligonucleotides you order.

RNA Chemistry and Proprietary Technologies of Sigma

Our RNA synthesis relies on the use of fast deprotection ribonucleoside phosphoramidites protected at the 2' position by a tert-butyldimethylsilyl (TBDMS) group and, at the 3' position, by a phosphoramidite.

RNA synthesis and deprotection are performed using Sigma's proprietary technologies ie. Ultra Fast Parallel Synthesis (UFPS) and Ultra Fast Parallel Deprotection (UFPD). The use of these technologies means higher coupling efficiency and faster deprotection, resulting in higher quality synthesis and faster turn-around times.

Specifications of siRNA Oligos

Quality control	All our siRNA oligos undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE or mass spectrometry analysis. Quantity is systematically validated by UV absorbance at 260 nm.
Purification	Fully deprotected and desalted Purified by RP-HPLC upon request
Delivered Quantity	2 OD (~10 nmol), 5 OD (~25 nmol), 10 OD (~50 nmol)
Length	19 to 27 mers
Bases	RNA DNA 2'OMe
Backbone	Phosphodiester bond
Labels and modifications	Amine, biotin, Cy-dyes, fluorescein, phosphate. Other labels available upon request
Format	Simplex siRNAs are delivered dry. Duplex siRNA oligos are delivered dry or liquid
Storage and stability	Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, Sigma recommends storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, siRNA oligos should be dried. Oligonucleotides with fluorescent labels should be protected from light. Sigma guarantees its oligonucleotides for six months, when stored under the above conditions.
Shipment	Shipped by express delivery, dry or liquid in individual, transparent tubes
Oligonucleotide Technical Data Sheet	Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, size, extinction coefficient and purification data. Additional gel images are provided for duplex confirmation upon request.
Services available upon request	Aliquoting Free design support
Pricing	Please contact your local sales representative
Ordering	On-line, by email or by fax

Related Products

Pre-designed siRNA

MISSION siRNA Human Druggable Genome

MISSION siRNA Rat Druggable Genome

MISSION siRNA Mouse Kinase Library

siRNA Literature for Download

Sales Brochure (626 Kb PDF)

siRNA Users Guide by Tuschl et al.

your favorite gene search

Service & Support

Ordering

Corporate

©2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. FLUKA is a trademark of Sigma-Aldrich GmbH, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Site Use Terms | Privacy