Custom siRNA Oligos

Attention: The following specifications apply to the USA and Europe. All other countries, click here.

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Features
Superior Manufacturing Capacity
RNA Chemistry and Proprietary Technologies of Sigma-Genosys
Specifications of siRNA Oligos
siRNA Pricing

Through the powerful science of RNA interference, successful gene silencing can be achieved with the use of synthetic, small interfering RNA (siRNA). By targeting these small, synthetic oligos, typically 19-23 bases in length against a specific gene of interest researchers are identifying gene function, elucidating pathways, and screening for potential new drug targets.
Features *New:* FAST siRNAs with ultra-fast turn-around time *New:* Modified nucleotides now available for siRNA products Deprotected, desalted and ready-to-use µg to multi-gram capacity Available in duplex or single-stranded form Purified by PAGE or RP-HPLC upon request Comprehensive QC and technical data sheet provided with each siRNA Rapid turn-around time and high throughput capacity back to top New siRNA Features FAST siRNAs Get your siRNAs faster than ever before. FAST siRNAs are produced in only 3 days. Available in 2 OD and 5 OD format. Modified nucleotides available for custom siRNAs! 2'OMe bases have been used successfully to increase the serum stability of siRNA duplexes and are of particular interest for in vivo applications of siRNAs. Try modified siRNAs for your next in vivo experiment. Unpurified siRNA is as effective as purified siRNA For successful knockdown of your gene of interest, order our siRNA (small interfering RNA) oligos. Our unpurified siRNA oligos are as effective in reducing gene expression as purified siRNA oligos, making them very cost effective. All our siRNA oligos are deprotected, desalted and ready-to-use. Our siRNA oligos are available at a fixed price per oligonucleotide (19 to 23 mers). Longer oligos and siRNA oligos containing modified nucleotides will be priced per base. Sigma produces siRNA under license from the Massachusetts Institute of Technology. back to top Superior Manufacturing Capacity With a capacity to synthesize over one million siRNA oligos per year, Sigma offers reliable delivery times, no matter how many oligonucleotides you order. back to top RNA Chemistry and Proprietary Technologies of Sigma Our RNA synthesis relies on the use of fast deprotection ribonucleoside phosphoramidites protected at the 2' position by a tert-butyldimethylsilyl (TBDMS) group and, at the 3' position, by a phosphoramidite. RNA synthesis and deprotection are performed using Sigma's proprietary technologies ie. Ultra Fast Parallel Synthesis (UFPS) and Ultra Fast Parallel Deprotection (UFPD). The use of these technologies means higher coupling efficiency and faster deprotection, resulting in higher quality synthesis and faster turn-around times. back to top
Specifications of siRNA Oligos
Quality control	All our siRNA oligos undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE or mass spectrometry analysis. Quantity is systematically validated by UV absorbance at 260 nm.
Purification	Fully deprotected and desalted Purified by RP-HPLC upon request
Delivered Quantity	2 OD (~10 nmol), 5 OD (~25 nmol), 10 OD (~50 nmol)
Length	19 to 27 mers
Bases	RNA DNA 2'OMe
Backbone	Phosphodiester bond
Labels and modifications	Amine, biotin, Cy-dyes, fluorescein, phosphate. Other labels available upon request
Format	Simplex siRNAs are delivered dry. Duplex siRNA oligos are delivered dry or liquid
Storage and stability	Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, Sigma recommends storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, siRNA oligos should be dried. Oligonucleotides with fluorescent labels should be protected from light. Sigma guarantees its oligonucleotides for six months, when stored under the above conditions.
Shipment	Shipped by express delivery, dry or liquid in individual, transparent tubes
Oligonucleotide Technical Data Sheet	Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm , MW, size, extinction coefficient and purification data. Additional gel images are provided for duplex confirmation upon request.
Services available upon request	Aliquoting Free design support
Pricing	Please contact your local sales representative
Ordering	On-line, by email or by fax
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Related Products Pre-designed siRNA MISSION siRNA Human Druggable Genome MISSION siRNA Rat Druggable Genome MISSION siRNA Mouse Kinase Library siRNA Literature for Download Sales Brochure (626 K PDF) siRNA Users Guide by Tuschl et al.
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