|
|
Sigma-Genosys
siRNA Oligos
|
|
|
|
Attention: Over the next few weeks, we will be updating our online order system and streamlining product specifications.
USA and Europe, Click Here
|
|
Through the powerful science of RNA interference, successful gene silencing can be achieved with the use of synthetic, small interfering RNA (siRNA). By targeting these small, synthetic oligos, typically 19-23 bases in length against a specific gene of interest researchers are identifying gene function, elucidating pathways, and screening for potential new drug targets.
|
Features
- Deprotected, desalted and ready-to-use
- µg to multi-gram capacity
- Available in duplex or single-stranded form
- Purified by PAGE or RP-HPLC upon request
- Comprehensive QC and technical data sheet provided with each siRNA
- Rapid turn-around time and high throughput capacity
back to top
Unpurified siRNA is as effective as purified siRNA
For successful knockdown of your gene of interest, order our siRNA (small interfering RNA) oligos. Our unpurified siRNA oligos are as effective in reducing gene expression as purified siRNA oligos, making them very cost effective. All our siRNA oligos are deprotected, desalted and ready-to-use.
Our siRNA oligos are available at a fixed price per oligonucleotide (19 to 23 mers). Sigma-Proligo produces siRNA under license from the Massachusetts Institute of Technology.
|
Yields, Labels and Transfections for siRNA Oligos
|
 |
back to top
|
Turn-Around Time for siRNA Oligos
|
 |
back to top
Superior Manufacturing Capacity
With a capacity to synthesize over one million siRNA oligos per year, Sigma-Proligo™ offers reliable delivery times, no matter how many oligonucleotides you order.
back to top
RNA Chemistry and Proprietary Technologies of Sigma-Proligo™
Our RNA synthesis relies on the use of fast deprotection ribonucleoside phosphoramidites protected at the 2' position by a tert-butyldimethylsilyl (TBDMS) group and, at the 3' position, by a phosphoramidite.
RNA synthesis and deprotection are performed using Sigma-Proligo's proprietary technologies ie. Ultra Fast Parallel Synthesis (UFPS) and Ultra Fast Parallel Deprotection (UFPD). The use of these technologies means higher coupling efficiency and faster deprotection, resulting in higher quality synthesis and faster turn-around times.
back to top
Specifications of siRNA Oligos
|
Quality control
|
All our siRNA oligos undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE or mass spectrometry analysis. Quantity is systematically validated by UV absorbance at 260 nm.
|
|
Purification
|
Fully deprotected and desalted
Purified by PAGE or RP-HPLC upon request
|
|
Length
|
19 to 23 mers
|
|
Bases
|
RNA (A, C, G or U)
DNA (A, C, G or T)
|
|
Backbone
|
Phosphodiester bond
|
|
Labels and modifications
|
Fluorescein, biotin and phosphate: 3' or 5'end. Other labels available upon request
|
|
Format
|
Single-strand and duplex siRNA oligos are delivered in dry form
|
|
Storage and stability
|
Although oligonucleotides are stable in solution at 4°C for up to 2 weeks, Sigma-Proligo recommends storage should be at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, siRNA oligos should be dried. Oligonucleotides with fluorescent labels should be protected from light. Sigma-Proligo guarantees its oligonucleotides for six months, when stored under the above conditions.
|
|
Shipment
|
Shipped by express delivery, dry in individual, transparent tubes
|
|
Oligonucleotide
Technical Data
Sheet
|
Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm , MW, size, extinction coefficient and purification data. Additional gel images are provided for duplex confirmation when applicable.
|
|
Services available upon request
|
• Aliquoting
• Free design support
|
|
Pricing
|
Please contact your local sales representative
|
|
Ordering
|
On-line, by email or by fax
|
back to top
| |
|
|
| |
|
|
| |
|
|
| |
| North America |
1 888 80 OLIGO
|
| Central / South America |
1 888 80 OLIGO
|
| Europe |
+0810 400 982
|
| Asia (not including Japan) |
+65 6873 2271
|
| Australia |
1 800 800 097
|
| New Zealand |
0 800 936 666
|
| Africa / Middle East / India |
+65 6873 2271
|
|
|
| |
|
|
| |
|
|
| |
| siRNA Literature for Download |
|
|
| |
|
|
| |
| Sales Brochure |
|
| |
| siRNA Users Guide by Tuschl et al. |
|
|
|
| |
|
|
|
back to top
|
|
