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Epigenetics

Epigenetics describes changes that are stable, but potentially reversible alterations in gene expression, that occur without permanent changes in DNA sequence and can still be passed on from generation to generation. Epigenetically controlled genes are activated or repressed without any change in DNA. Three central epigenetic mechanisms that play an essential role in gene regulation have been extensively studied by researches, including DNA methylation, histone modification, and RNA regulation. Our combined comprehensive Epigenetics portfolio offer high quality products to perform the techniques being utilized to study all the three central epigenetic mechanisms.
 

Chromatin immunoprecipitation (ChIP) kits

Quantitative detection of histone modifications is important to a better understanding of epigenetic regulation of cellular processes in normal or cancer tissues. The most widely used techniques to study how histone modifications and other DNA binding proteins, such as transcription factors, influence gene expression is called chromatin immunoprecipitation (ChIP) combined with qualitative polymerase chain reaction (qPCR). ChIP involves chemically crosslinking proteins to DNA sequences, which is followed by immunoprecipitation of the crosslinked complexes by using antibodies and beads to pull down the modified histone or other proteins of interest.  Below are some selected products for your research in histone modification. The most commonly studied and best understood histone modifications are acetylation, phosphorylation, methylation, and ubiquitination. Histone modifications regulate DNA transcription, repair, recombination, and replication, and can alter local chromatin architecture. We offer a wide range of kits for analyzing complex histone modifications patterns.

ChIP kits

Product No. Description Cart No.
17-10085 Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
17-10086 EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit
17-10077 Magna ChIP HT96
CHP2 Imprint® Ultra Chromatin Immunoprecipitation Kit
16-663 Magna ChIP™ Protein A+G Magnetic Beads

Histone Modification Assays

Product No. Description Cart No.
EPI010 SIRT2 Inhibitor Screening Assay Kit
EPI003 In-situ Histone Deacetylase (HDAC) Activity Flurometric Assay
CS1010 Histone Deacetylase Assay Kit, Fluorometric
EPI004 Histone Deacetylase 3 (HDAC3) Activity Assay Kit
EPI005 Histone Deacetylase 3 (HDAC3) inhibitor Screening Kit
EPI006 Histone Deacetylase 8 (HDAC8) Activity Assay Kit
EPI007 Histone Deacetylase 8 (HDAC8) inhibitor Screening Kit
17-344 H2A.X Phosphorylation Assay Kit (Flow Cytometry)

 

DNA Methylation

Bisulfite modification

Catalog # Description Add to cart
S7820-M CpGenomeTM Universal DNA Modification Kit
MOD50 Imprint® DNA Modification Kit
S7821-M CpGenome Universal Methylated DNA

Methylated DNA Enrichment, Isolation, Visualization and Quantification

Catalog # Description Add to cart
17-10035 CpGenome MethyQuestTM DNA Isolation Kit
DUO90806 Duolink
S7806 CpG WIZ® Prader – Willi/Angelman Methylation Amplification Kit
MDQ1 Imprint® Methylated DNA Quantification Kit

Whole genome amplification (WGA)

Catalog # Description Add to cart
WGA1 GenomePlex® Whole Genome Amplification (WGA) Kit
WGA2 GenomePlex® Complete Whole Genome Amplification (WGA) Kit
WGA3 GenomePlex® WGA Reamplification Kit
WGA4 GenomePlex® Single Cell Whole Genome Amplification Kit
SEQXE SeqPlex DNA Amplification Kit

 

RNA regulation

RNA Immunoprecipitation

Catalog # Description Add to cart
17-700 Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
RIP-12RXN Imprint® RNA Immunoprecipitation Kit
17-10521 EZ-Magna Nuclear RIP™ (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit

Transcriptome Amplification

Catalog # Description Add to cart
WTA2 Complete Whole Transcriptome Amplification Kit
WTA1 TransPlex® Whole Transcriptome Amplification Kit
SEQR SeqPlex RNA Amplification Kit