Epigenetics

Epigenetics describes changes that are stable, but potentially reversible alterations in gene expression, that occur without permanent changes in DNA sequence and can still be passed on from generation to generation. Epigenetically controlled genes are activated or repressed without any change in DNA. Sigma® has a wide portfolio of products to support Epigenetics research.

Epigenetics portfolio includes products for studying DNA methylation, chromatin modifications and RNA interference (RNAi). The portfolio includes kits for bisulphite modification, global DNA methylation quantification, chromatin-immunoprecipitation, and DNA amplification. Sigma also carries antibodies for gene regulation including histone modification, chromatin modification, transcription factors, transcription regulators, RNA regulation and nuclear proteins.

Sigma has leading functional genomics technologies including RNAi tools for gene silencing (siRNA, shRNA, miRNA) and cutting-edge systems for gene editing and gene knockout. Target genes for Epigenetics, stem cells and cancer are available in this collection. Our latest addition the CompoZr® Zinc Finger Nuclease (ZFN) gene editing and gene disruption technology which provides innovative solutions for eukaryotic and prokaryotic systems, has some existing kits for research in the above fields.

Besides this the portfolio also includes small molecules inhibitors, activators for methyltransferases, HATs and HDACs.

Products: Epigenetics Products SeqPlex WGA for Next Gen Sequencing ChIP Tested Antibodies  DNA Purification Imprint® Bisulfite DNA Modification Kit Imprint® ChIP Validated Antibodies Imprint® Chromoimmunoprecipitation (ChIP) Kit   Imprint® Ultra Chromatin Immunoprecipitation Kit Imprint® Global DNA Methylation Quantification Kit  Imprint® RNA Immunoprecipitation Kit Whole Genome Amplification (WGA) Bioactive Small Molecules for Research in Epigenetics Post Reaction  Tool Box: Your Favorite Gene Search Special Offers: 15% Off Mod50 and MDQ1 Technology Updates/Testimonials: Update on MOD50 Update on CHP1 Update on MDQ1

Applications Center: Protocols    - Cold Spring Harbor Laboratory Protocols    - Protocols on SciClip site Literature References Videos Life Science Application Center Learning Center: Contact Us Epigenetics Overview Technologies of Interest Epigenetic Control DNA Methylation and Transcription Repression Chromatin Remodeling Modified Histones Histone Modifying Enzymes Formation of Nucleosomes DNA Compaction Metastasis associated 1 (Gene MTA1) Homo sapiens Mechanisms of Action     Related Topics: Functional Genomics Gene Regulation Labware   Core Bioreagents

Information & News

 

Imprint® Methylated DNA Quantification technology A rapid and reliable method to measure global DNA methylation shifts in about half of a day. Fast - The total protocol can be completed in about 4 hours Flexible - 8 well assay strips may be used for high-throughput applications or manual analysis; genomic DNA from cultured cells, tissues, plasma, or other body fluid samples may be used Convenient - This simple protocol uses a familiar ELISA-like procedure:  colorimetric quantification and no radioactivity; low DNA input from 10-200 ng Complete - Includes methylated control DNA and all necessary reagents except water Sensitive - Detection limit is 5 ng of fully methylated genomic DNA (we used human in our tests).