Epigenetics

Imprint® Chromatin Immunoprecipitation Kit

The Fastest and Most Convenient ChIP Kit Available!

Sigma combines speed and convenience with performance in the Imprint Chromatin Immunoprecipitation Kit (CHP1). The Imprint ChIP Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2). The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.


Did you know... Graphic
  • Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
  • Sensitive—As few as 20,000 cells required for each ChIP sample
  • Convenient—Fewest steps of any available ChIP protocol
  • Flexible—Protocols for cells or tissue, and convenient
    strip-well format for high-throughput applications
  • Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology


View the Chromatin IP (ChIP) Troubleshooting Questions

View the poster Rapid and easy method for Chromatin IP (ChIP) analysis of metastatic cells
(446 KB PDF).

View the technical article ChIP Protocol To Rapidly Characterize DNA-Protein Interactions


Figure 1 - Protocol Time Graph

Figure 1. Comparison of time required for protocol completion from fixation through purification using different Chromatin Immunoprecipitation kits. Each ChIP experiment was carried out using each manufacturers recommended protocol and total required times were compared.

Figure 2: One day is sufficient to acquire ChIP enriched DNA using Sigma's Imprint ChIP protocol. ChIP with antibodies against mouse IgG, H3K9ac, RNAP and H3K27me3 was completed on 10,000 and 100,000 SW480 cells per reaction. A fraction (1%) of the sonicated chromatin was set aside as 'input' DNA before the antibody affi nity manipulations. DNA was purified following cross-link reversal. The resulting enriched DNA was probed for GAPDH using specific primers by qPCR. Percent input was calculated by 100 x 2^(Ctadjusted Input — CtEnriched). Input DNA Ct was adjusted from 1% to 100% equivalent by subtracting 6.644 Cts or Log2 100.

Figure 3 - RNA Pol2 Antibody Percent Input Profile Graph Figure 4 - H3K9ac Antibody Percent Input Profile Graph
Figures 3 and 4. A highly sensitive ChIP assay. HeLa cells were counted, fixed, and immunoprecipitated according to the Imprint CHP1 bulletin with optional High Sensitivity method. ChIP assays were performed using anti-H3K9ac (H9286) and kit provided antibodies against: human RNA Polymerase II and non-specific mouse IgG. To evaluate ChIP enrichment, SYBR qPCR was conducted targeting the GAPDH promoter region (highly expressed house-keeping gene). Percent Input describes the amount of DNA with and without antibody selection. For these antibodies, apparent enrichment increased with fewer cells per reaction from diminished non-specific pull-down.

Ordering Information

Product No. Product Name
CHP1-24RXN
CHP1-96RXN
Imprint Chromatin Immunoprecipitation Kit

Products to Support the Epigenetics Workflow using this kit

DNA extraction and modification

Prod No. Product Name
G1N10 GenElute™ Mammalian Genomic DNA Miniprep Kit
NA2010 GenElute Blood Genomic DNA Kit
G2N10 GenElute Plant Genomic DNA Miniprep Kit
MOD50 Imprint DNA Modification Kit

DNA-protein interaction & ChIP validated antibodies

Prod No. Product Name
A5463 Anti-Acetylated Proteins antibody produced in rabbit
H0913 Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse
H2663 Monoclonal Anti-Histone Deacetylase 2 (HDAC2) antibody produced in mouse
H7162 Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit
H7789 Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit
H9161 Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit
H9286 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit
R4279 Anti-RbAp46, C-terminal antibody produced in rabbit

MISSION® shRNA for functional genomic studies

Prod No. Product Name
SH3011 MISSION shRNA Human Gene Family Set, Bacterial Glycerol Stock Epigenetic Regulators
SH3021 MISSION shRNA Human Gene Family Set, DNA Epigenetic Regulators
SH3031 MISSION shRNA Human Gene Family Set, Lentiviral Particles Epigenetic Regulators

 

Imprint ChIP Validated Antibodies

Successful chromatin immunoprecipitation requires an effective, specific, and high quality antibody. Not all antibodies work in ChIP as the antigen epitope may be blocked or altered once cross-linked. Sigma's Imprint brand of antibodies are validated in ChIP application and lot tested each time for successful chromatin immunoprecipitation experiment. For a complete list of ChIP validated antibodies please click here.

 

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