MISSION^® siRNA Universal Negative Controls are an essential component to any siRNA experiment. Using a Negative siRNA control allows the researcher to create a baseline for mRNA knockdown efficiency. MISSION siRNA Universal Negative Controls have been tested in human, rat, and mouse cells. All have carefully designed to have no homology to known gene sequences.

Benefits of MISSION Universal Negative siRNA Control:

• Universal Negative Controls designed for use in Human, Mouse, or Rat cells
• Novel design method used to ensure no homology to all Mature and Predicted RefSeq mRNA sequences.
• Contain no known immune-response motifs¹
• Validated with Agilent 40K human gene arrays to ensure no significant nonspecific gene interactions
• Functionally validated to show no immune response via qRT PCR
• Available in convenient 5x1nmol aliquots or 1x1nmol package
• Two different Universal Negative Control siRNA sequences

For most siRNA experiments we recommend MISSION siRNA Universal Negative Control 1. Universal Negative Control 2 can also be used as an additional control or as an alternative to Universal Negative Control 1.

References:
1. Judge, A.D., Sood, V., Shaw, J.R., Fang, D., McClintock, K., and MacLachlan, I. (2005) Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 23, 457.
2. Hornung, V. et al. (2005) Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 11, 263.