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Functional Genomics & RNAi
Microinjection of dsRNA Protocol
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The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in an exclusive partnership with Sigma-Aldrich. Each month, 2 new protocols will be featured, each with a list of products available from Sigma-Aldrich. |
| View the protocol on BioSupplyNet.com. |
Selected Protocol from "RNAi, A Guide to Gene Silencing", Gregory J. Hannon.
Mammalian RNA Interference, Thomas Tuschl (Chapter 13, pg. 280-288) |
Buy this book, Z701130 |
| Microinjection of dsRNA into Mouse Oocytes and Early Embryos |
| ABSTRACT |
| This protocol describes how to introduce a double-stranded RNA (dsRNA) of choice into mouse oocytes or fertilized one-cell embryos by microinjection. For collection of mouse oocytes and early embryos, see Collection of Mouse Oocytes for RNAi and Collection of Early Mouse Embryos for RNAi. It is advisable to confirm knock-down of the gene of interest by RT-PCR (see RNA Isolation and RT-PCR from dsRNA-treated Mouse Oocytes and Early Embryos). |
| Products Available for this Protocol |
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Buffers, Solutions, and Reagents |
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| NaCl |
S3014 |
Sodium chloride |
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| KCl |
P9541 |
Potassium chloride |
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| K2HPO4 |
P9666 |
Potassium phosphate dibasic trihydrate |
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| MgSO4 |
M2643 |
Potassium phosphate dibasic trihydrate |
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| CaCl2 |
21115 |
Calcium chloride solution |
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| NaHCO3 |
S3817 |
Sodium bicarbonate, Biotechnology Performance Certified, cell culture tested |
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| Sodium lactate |
L4263 |
Sodium DL-lactate solution |
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| Sodium |
483745 |
Sodium |
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| DMSO |
D8418 |
Dimethyl sulfoxide for molecular biology |
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| Streptomycin |
S9137 |
Streptomycin sulfate salt |
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| L-glutamine |
G6392 |
L-Glutamine cell culture tested, γ-irradiated |
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| EDTA |
E7889 |
Ethylenediaminetetraacetic acid disodium salt solution |
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| Bovine serum albumin (BSA) |
B6917 |
Albumin from bovine serum, for molecular biology, Non-acetylated, lyophilized powder |
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| Gentamicin |
G1272 |
Gentamicin solution |
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| Phenol Red |
P3532 |
Phenol Red, powder, cell culture tested |
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| Polyvinylpyrrolidone (PVP) |
P5288 |
Polyvinylpyrrolidone, for molecular biology, nucleic acid hybridization tested |
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| Glucose |
G5400 |
D-(+)-Glucose |
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| Penicillin G |
P3032 |
Penicillin G sodium salt |
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| IBMX (3-isobutyl-1-methylxanthine) |
I7018 |
3-Isobutyl-1-methylxanthine, SigmaUltra |
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| HEPES (pH 7.2) |
51558 |
HEPES buffered saline, for molecular biology, 2× concentrate |
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| Sodium pyruvate |
P5280 |
Sodium pyruvate, powder, cell culture tested, insect cell culture tested |
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| Bicarbonate-free minimal essential medium (Earle's salts) |
E7510 |
10 ×, Without sodium bicarbonate, sterile-filtered, liquid, cell culture tested |
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| Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest. |
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