shRNA

Troubleshooting

 
MISSION shRNA

What should I do if I have trouble obtaining high yields of plasmid DNA?
Generally, high yields of DNA can be achieved with much of the collection; however, viral-based vectors are known to be somewhat difficult to prep. If particular clones are troublesome, we recommend streaking the bacterial stocks on LB/carbenicillin plates to isolate a single colony and DNA purification with GenElute™ Endotoxin free Midi (Product Code NA0200) or GenElute™ Endotoxin free HP Maxi (Product Code NA0400S) prep kits.

 


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For questions about the library, pricing and quotes or other concerns, please e-mail us at: MISSIONRNAi@sial.com.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.

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