Lentiviral Titer by Limiting Dilution Protocol (63.6 Kb PDF)
Developed by Sigma® MISSION® RNAi Team
Lentiviral Transduction Protocol (69.0 Kb PDF)
Developed by Sigma MISSION® RNAi Team
Lentiviral Transduction Protocol without Puromycin
Developed by Sigma MISSION® RNAi Team
Lentiviral Transduction of Suspension Cells—Spinoculation Protocol
Adapted from Dr. Una O'Doherty, University of Pennsylvania School of Medicine
Lentiviral Transduction Protocol for Human Embryonic Stem Cells
Jennifer Moore, Ph.D., Alana Toro-Ramos, M. Sc., and Rick Cohen, Ph.D. Rutgers University Stem Cell Research Center
Optimal Cell Density Determination (cells/ml) for Transduction in Osteosarcoma Cells, KHOS, Using MISSION® LentiExpress™ Optimization Plate
Provided by Dr. Zhenfeng Duan, Harvard Medical School
Protocol for Screening Osteosarcoma Cells, KHOS, Using MISSION® LentiExpress™ Kinase Panel
Provided by Dr. Zhenfeng Duan, Harvard Medical School
Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit
Provided by Sigma MISSION® RNAi Team
Titer by p24 Assay - ELISA for the p24 Viral Coat Protein
Protocol provided by Sigma MISSION® RNAi Team
Determining Relative Transduction of Adherent Cells using MISSION® TurboGFP™ Transduction Particles
Protocol provided by Sigma MISSION® RNAi Team
Preparation of the Lentiviral Transduction Particles using Packaging Plasma Mix
Protocol provided by Sigma MISSION® RNAi Team
Protocol for Transducing Bone Marrow Derived Macrophages (BMDM) using MISSION® ExpressMag™ Super Magnetic Kit
Protocol provided by Sigma MISSION®RNAi Team
Optimization of Antibiotic Selection Using a Cytotoxicity Profile
Protocol provided by Sigma MISSION®RNAi Team
Cold Spring Harbor Laboratory Press Protocols
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