Functional Genomics & RNAi

MISSION® Protocols


MISSION RNAi


Lentiviral Titer by Limiting Dilution Protocol (PDF)
Developed by Sigma® MISSION® RNAi Team

Lentiviral Transduction Protocol (PDF)
Developed by Sigma MISSION® RNAi Team

Lentiviral Transduction of Suspension Cells—Spinoculation Protocol
Adapted from Dr. Una O'Doherty, University of Pennsylvania School of Medicine

Lentiviral Transduction Protocol for Human Embryonic Stem Cells
Jennifer Moore, Ph.D., Alana Toro-Ramos, M. Sc., and Rick Cohen, Ph.D. Rutgers University Stem Cell Research Center

Protocol for Screening Osteosarcoma Cells, KHOS, Using MISSION® LentiExpress™ Kinase Panel
Provided by Dr. Zhenfeng Duan, Harvard Medical School

Determining Relative Transduction of Adherent Cells using MISSION® TurboGFP™ Transduction Particles
Protocol provided by Sigma MISSION® RNAi Team

Preparation of the Lentiviral Transduction Particles using Packaging Plasma Mix
Protocol provided by Sigma MISSION® RNAi Team

Protocol for Transducing Bone Marrow Derived Macrophages (BMDM) using MISSION® ExpressMag™ Super Magnetic Kit
Protocol provided by Sigma MISSION®RNAi Team

Optimization of Antibiotic Selection Using a Cytotoxicity Profile
Protocol provided by Sigma MISSION®RNAi Team

Cold Spring Harbor Laboratory Press Protocols

 


Contact Us


For questions about the library, pricing and quotes or other concerns, please e-mail us at: MISSIONRNAi@sial.com.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.

Back to Top
RNAi Homepage