Technical Workshop

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	Dates Location Overview Lecture and Laboratory Topics	Speakers Feedback from Attendees Accommodations Register Now! Download Workshop Flyer (584 Kb PDF)

Dates

December 8-10, 2009.

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Location

Sigma^® Life Science and High Technology Center
St. Louis, Missouri
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Sigma is now able to bring the workshop to you! For more information about bringing the workshop to your institution please contact TechnicalWorkshops@sial.com

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Overview (ZFN)

Zinc finger nucleases (ZFNs) enable manipulation of the genome with unprecedented ease and precision. ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks (DSBs) in DNA at user-specified locations. DSBs are important for site-specific mutagenesis in that they stimulate the cell’s natural DNA-repair processes, namely homologous recombination and non-homologous end joining. Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, including somatic cell lines, with targeted gene deletions (knockouts), integrations, or modifications. Featured lectures will provide in depth discussion on the fundamentals of ZFNs as well as cutting edge applications for targeted gene knockout, integration and tagging. After mastering ZFN technology, registrants will have all of the tools they will need to modify genes in standard tumor cell lines, primary cells and embryonic stem cells. Registrants should be familiar with basic mammalian cell culture techniques.

 Targeted Genome Editing Using Zinc Finger Nucleases Lectures
Introduction to ZFN Technology
ZFN Design
Gene Editing and Delivery
Double-strand Break Repair
Gene Knockout and Validation
Gene Insertion and Tagging
Donor Design
Dilution Cloning and Genotyping
Transgenics
Therapeutics

Targeted Genome Editing Using Zinc Finger Nucleases Labs
Nucleofection
Nucleotide Mismatch Assay
Junction PCR
RFLP Assay

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Lecture and Laboratory Topics

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Speakers

Past Speakers
Mikael Kubista, Ph.D. Founder TATAA Biocenter
Hakim Djaballah, Ph.D. Director, HTS Core Facility at MSKCC
Rick Cohen, Ph.D. Director of hESC Core Facility at Rutgers
Aubrey Thompson, Ph.D. Professor of Biochemistry and Molecular Biology at Mayo Clinic
Richard Hotchkiss, M.D. Professor of Anesthesiology, Medicine and Surgery at Washington University
Michael McManus, Ph.D. Assistant Professor at UCSF Diabetes Center
Sheila Stewart, Ph.D. Assistant Professor Cell Biology and Physiology at Washington University

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Feedback from Attendees

"Absolutely awesome. Everyone at Sigma is very receptive and ready to assist with all needs. I would highly recommend this workshop to anyone in the field. I am very glad that I had this opportunity." — Laia Gómez Baldó, Institute Català d'Oncologia, Spain

"The Sigma-Aldrich RNAi workshop included a nice balance of theoretical and practical information. In particular, the hands-on components of the workshop showed me how easily I could incorporate siRNA and shRNA approaches into my research projects. The Sigma-Aldrich RNAi team was knowledgeable, helpful, and above all, friendly and accessible." — Jon Fisher, St. Louis University Biology Department, Missouri, USA

"I just wanted to thank you for the hospitality you have shown us during our stay in St. Louis. It was a very pleasant experience and it felt very good to know that there are people that not only care about the science but also about fellow human beings. The workshop was very nicely arranged, if there were any hiccups, I did not see any. The crew was also very helpful and friendly, that counts a lot for me..." — Phiyani Lebea, at CSIR Biosciences, South Africa

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Accommodations

The city of St. Louis offers a variety of convenient transportation and accommodation options. Lambert St. Louis International Airport (STL) is connected to downtown St. Louis by a major interstate (I-70) as well as a convenient Metrolink system that directly connects the airport terminal to multiple downtown stops and locations. Once in St. Louis, we strongly recommend staying at the right to Hilton at the Ballpark. Sigma has negotiated a special low rate for all of the RNAi workshop participants. In addition, a free shuttle will take all participants from the Hilton each morning to the Sigma Life Science and High Technology Center and then return them at the end of each day. This allows participants to focus on the RNAi course and not worry about scheduling transportation.

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