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Functional Genomics & RNAi

miRNA Research Tools

Complete microRNA Solutions for Screening, Identification and Validation

microRNA (miRNA) is a functional RNA molecule that is not translated into a protein. MicroRNAs are involved in many cellular processes, gene regulation and disease states such as cancer. Sigma is proud to offer a variety of tools that enhance miRNA research.



Screening
MISSION miRNA Human Mimics

mirPremir™ microRNA Isolation Kit

  • Optimized for purification of miRNA
  • Fast 30 minute protocol
  • Highly pure miRNA with no large RNA

MISSION Synthetic and Lentiviral miRNA Inhibitors

  • Designed using a proprietary algorithm based upon the Tough Decoy (TuD) design1,2
  • Allows for potent inhibition of the desired miRNA
Identification
MISSION miRNA Target ID Library
  • Enables functional identification of biologically relevant mRNA targets regulated by microRNAs
  • Greater biological relevance compared to miRNA target prediction algorithms
  • Human cDNA library cloned downstream of a fusion construct allowing positive and negative selection
Validation
MISSION 3’UTR Lenti GoClone
  • miRNA target validation using a luciferase reporter construct
  • Over 10,000 3’UTRs in lentiviral vector
  • Enhanced luciferase reporter for more efficient validation of functional miRNA targets at the 3’UTR region of gene

References

  1. Haraguchi, T., et al., Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acids Res., 37, (2009).
  2. Haraguchi, T, et al. A potent 2’-O-methylated RNA-based microRNA inhibitor with unique secondary structures. Nucleic Acids Res. 2012 Apr;40(8):e58. Epub 2012 Jan 17.