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Life Science > Functional Genomics & RNAi > miRNA Research Tools > MicroRNA Mimics |
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MISSION miRNA mimics are small, double-stranded RNA molecules, designed to mimic endogenous mature miRNA molecules when transfected into cells. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. MISSION miRNA mimics utilize a proprietary design to minimize potential sense strand off-target effects.
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miRNA Ordering Information Easily find your MISSION miRNA target by inserting your gene of interest into the search box below. To search by miRNA name or accession number, please use our main search box at the top of this page.
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MISSION microRNA mimics significantly reduce known gene target express. HeLa cells were co-transfected with MISSION miRNA mimics and psi-CHECK2 Dual Luciferase Reporter Construct (Promega), containing corresponding miRNA target sequences. Constructs contained full length 3'UTR sequence specific miRNA sequences (100% complementary to the mature miRNA). The MISSION miRNA mimic negative controls are non-human miRNAs, predicted to not target the human genome/transcriptome.
MISSION miRNA mimic design was tested to measure possible passenger strand off-target effects. Passenger strands of the miRNA mimic were either designed by traditional siRNA design (siDesign) with (+) or without (-) chemical modification, or by MISSION miRNA Mimic Design with (+) or without (-) chemical modification. Reporter constructs contained targets that were ~95% complementary to the passenger strand sequence. The MISSION miRNA mimcs use a proprietary design and chemical modification to effectively reduce off-target effects. Selected citations for miRNA mimics
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