MISSION^® Human miRNA Mimics

MISSION miRNA mimics are small, double-stranded RNA molecules, designed to mimic endogenous mature miRNA molecules when transfected into cells. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. MISSION miRNA mimics utilize a proprietary design to minimize potential sense strand off-target effects.

• Human miRNA mimic library based on miRBase ver. 13
• Optimized and ready for transfection
• Novel MISSION miRNA mimic design has been functionally tested for knockdown efficiency against natural miRNA targets
• Unique MISSION miRNA mimic design significantly reduces possible sense strand off target effects
• Available as a whole human library and individual miRNA targets

Available Products

• Complete library is available in 96 well format. (Two outside columns, 1 and 12, have been left empty for the addition of controls)
• 985 individual miRNA mimics are available on the shelf and typically ship within one day of purchase
• 2 non-targeting miRNA human controls
• Custom target sequence design available

miRNA Ordering Information

Easily find your MISSION miRNA target by inserting your gene of interest into the YFG search box below.

 

Custom miRNA inquiries please contact your local Sigma-Aldrich® Sales Representative or dial (800) 234-5362 choose option 3: ext. 1831.

Product listings for miRNA mimics

Functionally Validated

MISSION microRNA mimics significantly reduce known gene target express. HeLa cells were co-transfected with MISSION miRNA mimics and psi-CHECK2 Dual Luciferase Reporter Construct (Promega), containing corresponding miRNA target sequences. Constructs contained full length 3'UTR sequence specific miRNA sequences (100% complementary to the mature miRNA). The MISSION miRNA mimic negative controls are non-human miRNAs, predicted to not target the human genome/transcriptome.

Reduce Sense Strand Off-Target Effects

MISSION miRNA mimic design was tested to measure possible passenger strand off-target effects. Passenger strands of the miRNA mimic were either designed by traditional siRNA design (siDesign) with (+) or without (-) chemical modification, or by MISSION miRNA Mimic Design with (+) or without (-) chemical modification. Reporter constructs contained targets that were ~95% complementary to the passenger strand sequence. The MISSION miRNA mimcs use a proprietary design and chemical modification to effectively reduce off-target effects.

Selected citations for miRNA mimics

1. Collino F., Deregibus M., Bruno S., Sterpone L., Aghemo G., et al. (2010) Microvesicles Derived from Adult Human Bone Marrow and Tissue Specific Mesenchymal Stem Cells Shuttle Selected Pattern of miRNAs. PLoS One 5:e11803.
2. Schultz, N. et al. Off-target effects dominate a large-scale RNAi screen for modulators of the TGF-β pathway and reveal microRNA regulation of TGFBR2. Silence 2011, 2:3

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