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Functional Genomics & RNAi

MISSION® Synthetic miRNA Inhibitors

MISSION synthetic miRNA inhibitors (S-TuD)1 are small, double-stranded RNA molecules that regulate gene expression by binding to and inhibiting a specific mature miRNA. The miRNA inhibitors were designed using a proprietary algorithm, developed in collaboration with Dr. Hideo Iba and Dr. Takeshi Haraguchi from the University of Tokyo1, and the mature miRNA sequence information from miRBase. The algorithm computes all possible sequence parameters, selects the one predicted to best maintain the TuD structure, providing maximum miRNA recognition and binding. Each synthetic TuD (S-TuD) is stabilized by incorporating 2’-O-methylated nucleotides, and provides two miRNA binding sites. Optimal miRNA inhibition is provided after transfection due to the robust secondary structure of the inhibitor that is based upon the synthetic 'Tough Decoy' (TuD)2 molecule.

Advantages

  • Published technology
  • Long lasting inhibition at very low nmol concentrations
  • Excellent resistance to cellular nucleases
  • Structure provides potent inhibition of the desired miRNA
  • Custom synthesis available for a variety of species

Product Offering

Synthetic miRNA inhibitors are available for human, mouse or custom species.  Two negative controls; one Arabidopsis thaliana sequence and one Caenorhabditis elegans sequence are available.

Product No. Product Description Quantity*
HSTUDxxxx
Individual human miRNA inh. 5 nmol
MSTUDxxxx Individual mouse miRNA inh. 5 nmol
NCSTUD001-002 Negative controls 5 nmol
Inquire Custom miRNA inhibitor 20 nmol

* Quantity provided is sufficient to bind the value listed of target miRNA

Browse a complete list of human or mouse Synthetic miRNA inhibitors.

How to Order

Individual miRNA inhibitors
Easily find your synthetic miRNA inhibitor by inserting the name, mature accession # or sequence of the miRNA of
interest into the search box above and add the selected miRNA inhibitor to your cart.

Controls
Negative controls: Arabidopsis thaliana (NCSTUD001) and Caenorhabditis elegans (NCSTUD002)

Custom miRNA inhibitors

MISSION Synthetic microRNA Inhibitors Significantly Reduce Target microRNA Activity

Click on image for larger view.


HeLa cells were co-transfected with MISSION Synthetic microRNA Inhibitors and psi-CHECK2 Dual Luciferase Reporter Construct (Promega), containing corresponding miRNA target sequences, 100% complementary to the mature miRNA, downstream from Renilla. Increased Renilla expression is observed when endogenous miRNA activity is inhibited. The MISSION miRNA inhibitor negative control targets Arabidopsis miRNA 416, which is not expressed in human cells.


MISSION Synthetic microRNA Inhibitors inhibit target microRNA activity across a wide concentration range equivalently. HeLa cells were co-transfected with MISSION synthetic microRNA Inhibitors and a psi-CHECK2 Dual Luciferase Reporter containing 2 copies of the sequence 100% complementary to the corresponding mature miRNA target sequence. Dual luciferase reporter assays (Promega) were performed 48 hours post-transfection. Increased Renilla expression is observed when endogenous miRNA activity is inhibited. A MISSION miRNA inhibitor targeting Arabidopsis miRNA 416, which is not expressed in human or mouse cells, was used as a negative control. Optimum inhibitor concentration should be determined empirically for each specific experimental situation.


MISSION Synthetic microRNA Inhibitors inhibit microRNA activity equivalently, or more effective than competing technologies. HeLa cells were co-transfected with an inhibitor from each company and a psi-CHECK2 Dual Luciferase Reporter with 2 perfectly complementary copies of the mature hsa-miR-191-5p target sequence inserted downstream from Renilla luciferase. Dual luciferase reporter assays (Promega) were performed 48 hours post-transfection. Increased Renilla expression is observed when endogenous miRNA activity is inhibited.


References

  1. Haraguchi, T, et al. A potent 2’-O-methylated RNA-based microRNA inhibitor with unique secondary structures. Nucleic Acids Res. 2012 Apr;40(8):e58. Epub 2012 Jan 17.
  2. Haraguchi, T., et al., Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acids Res., 37, (2009).