mirPremier™ microRNA Isolation Kit
More rapid, more pure, less hazard, less hands on.
The mirPremier microRNA Isolation Kit complements the already robust MISSION® RNAi product portfolio which includes a broad choice of MISSION siRNA and shRNA products and services such as libraries, mRNA detection reagents, antibodies and AQUA peptides for protein level detection as well as an entire workflow of supporting reagents (cell culture, cell biology compounds and assays, transfection reagents, and much more). For more information, visit the Functional Genomics & RNAi homepage.
MicroRNA (miRNA) are a class of small RNA molecules, about 21 nucleotides in length that regulate gene expression in a variety of ways, including translational repression, mRNA cleavage and deadenylation. Developed by Sigma, the mirPremier microRNA Isolation Kit is a new member of the MISSION® RNAi product family that provides a rapid and efficient method for purifying and enriching miRNA along with other small RNAs, allowing researchers to obtain high-quality miRNA directly from cells or tissues as rapidly and simply as a total RNA prep.
Using mirPremier microRNA Isolation Kit, researchers can purify miRNA/small RNA from diverse biological sources, including mammalian cell cultures, animal tissues, microbial cultures and plant tissues (Figure 1). mirPremier microRNA Isolation Kit also allows researchers to purify miRNA/small RNA with high yield from "difficult-to-extract" tissues, such as grape leaf (Figure 2). In addition, the kit can be used to isolate total RNA if messenger RNA or other large RNAs are of interest.
Timely extraction of miRNA from cells or tissues is critical for downstream applications. mirPremier™ microRNA Isolation Kit provides a robust protocol that enables scientists to purify miRNA within 30 minutes, making it an ideal solution for rapid preparation of miRNA (Figures 3 and 4).
Unlike other miRNA purification methods, mirPremier microRNA Isolation Kit yields high-purity miRNA/small RNA, containing no detactable large RNA (Figure 5).
The mirPremier microRNA Isolation Kit employs a novel purification chemistry to isolate miRNA and other small RNAs in a simplified and streamlined manner, without using hazardous organic extractions such as phenol and chloroform.
Like other Sigma life science products, the mirPremier microRNA Isolation Kit is backed by the quality production standards, customer service, technical support, and other extensive offerings that make Sigma the most trusted name in life science technology.