Functional Genomics & RNAi

Introducing MISSION® Synthetic and Lentiviral miRNA Inhibitors

Elucidate miRNA Function with our New miRNA Inhibitors

Inhibition of microRNAs is essential to studying their function. Sigma® Life Science offers a collection of individual microRNA inhibitors which are designed using a proprietary algorithm based upon the Tough Decoy (TuD) design.1 Each miRNA inhibitor was designed using the mature miRNA sequence information from miRBase and encompasses two miRNA binding sites. The MISSION miRNA inhibitors are based upon the work of Dr. Takeshi Haraguchi and in collaboration with Dr. Hideo Iba, from the University of Tokyo.


Choose between Synthetic and Lentiviral formats

Synthetic miRNA Inhibitors

  • 2’-O-methylated RNA duplexes with a robust secondary structure for optimal miRNA inhibition2
  • Long lasting inhibition at very low nmol concentration
  • Custom synthesis available for a variety of species

Lentiviral miRNA Inhibitors

  • Allows for potent inhibition of desired miRNA
  • Efficient delivery of the inhibitor into a wide variety of cell types
  • Enables long-term inhibition without repeat transfections
  • Constructs have been cloned and sequence verified to ensure a match to the target miRNA

Read the Papers Behind the Technology from Dr. Iba's Lab

 

  1. Haraguchi, T., et al. Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acids Res., 37, (2009).



  2. Haraguchi, T., et al. A potent 2’-O-methylated RNA-based microRNA inhibitor with unique secondary structures. Nucleic Acids Res., 40, (2012).

Dr. Hideo Iba
Department of Microbiology and Immunology
The Institute of Medical Science
University of Tokyo