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RNAi FAQs
Choosing an RNAi Format | ||
I am new to RNAi work. Which should I choose, siRNA or shRNA?
Both methods provide viable knockdown efficiency, to better help you choose please refer to the table below.
Choosing the Appropriate RNAi Effector for your Application
| siRNA | shRNA, Transfected plasmid | shRNA, Lentivirus-Delivered | |
|---|---|---|---|
| Cell Lines (actual efficacy highly cell type specific) | |||
| Immortalized/Cancer | XXX | XXX | XXX |
| (Embryonic) Stem | X | X | XXX |
| Primary | X | X | XXX |
| Screening | |||
| Arrayed, (single) | XXX | XX | XXX |
| Pooled, multiple genes / genome | - | - | XXX |
| Pooled, single gene | XXX | X | X |
| in vivo (routes) | |||
| Intratumoral | XXX | XXX | XXX |
| ex vivo, implantation | X | X | XXX |
| Cranial | X | X | XX |
| Peritineum | X | X | XX |
| Muscle | XX | XX | XX |
My gene of interest is very stable. Which RNAi format do I choose? Both methods provide viable knockdown efficiency, to better help you choose please refer to the table below.
RNAi effector Features
| siRNA | shRNA, Transfected plasmid | shRNA, Lentivirus-Delivered | |
|---|---|---|---|
| Silencing Duration | Transient (48hrs to 1wk) | Transient (48hrs to 1wk), or Stable, long-term | Stable, long-term |
| mRNA silenced | 6 - 24 hours after transfection | 24 - 48 hours after transfection | 48 hours after transduction |
| Protein silenced | 48 hours to 5 days post-transfection | 48 hours to 5 days post-transfection | 72 hours to 6 days post-transduction |
| Ease of Use | Simple | Moderate | Simple |
| What comes Standard | ~10 nmoles, desalted | 1µg, TE buffer | 200 µl of 1 x 10^6 TU/ml |
| Custom Options | Yes | Yes | Yes |
For additional questions regarding our siRNA and shRNA product lines, please select one of the links below: