MISSION Application Data

Sigma-Aldrich® is committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mailing MissionRNAi@sial.com.

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Posters – MISSION R&D
Posters – External Researchers
Selected Data Figures
Publications Citing MISSION^® TRC Clones

Posters – MISSION R&D

Posters – External Researchers

Lentiviral shRNA Screen of Multidrug Resistant Associated Genes Identifies PRP-4 as a New Regulator of Chemoresistance in Human Ovarian Cancer (635 Kb PDF)
Zhenfeng Duan, M.D. Ph.D. – Harvard Medical School
shRNA Phenotypic Screen To Identify Gene Targets Contributing to Androgen Independent Prostate Cancer Cell Growth (989 Kb PDF)
Daniel Gioeli Ph.D. – University of Virginia
Mesenchymal Stem Cells: Cell Death or Survival? Interaction with Other Stress Pathways (887 Kb PDF)
by Oliver L. and Vallette FM, Center for Cancer Research Nantes - Angers INSERM UMR 892, University of Nantes, France

Selected Data Figures

Data type	Format	Gene Targets	Cell Lines	Detection Level	Provided by
Long Term, up to 1 yr, Gene Silencing with shRNA	Viral Particles	ARGHDIA	A549	mRNA	Sigma-Aldrich R&D
Gene Target Validation	Viral Particles, Transduction	CDH1	Human colorectal cancer cell line (coll5)	Protein	Elena Sancho, PhD and Eduard Batlle, PhD Barcelona Institute for Research in Biomedicine
Stable Cell Line/ Signaling Pathway	Viral Particles, Transduction	ZFP36 (TTP)	A549	mRNA & Protein	Kathleen Smoak, PhD and John A Cidlowski, PhD Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health
Efficacy, Specificity and Efficiency	Viral Particles, Transduction	p38α, p38γ, JNK2, MAP3K4, JAK1, AKT1, AKT2, AKT3	Primary human astrocytes, A549, HepG2, HCT116, Panc1, HeLa	mRNA	Sigma-Aldrich R&D
Primary Astrocyte Cells (Neuronal Cells)	Viral Particles, Transduction	p38α, p38γ, JNK2, MAP3K4	Primary human astrocytes	mRNA	Sigma-Aldrich R&D
Specificity in Targeting Isoforms	Viral Particles, Transduction	AKT1, AKT2, AKT3	A549	mRNA	Sigma-Aldrich R&D
Gene Target Validation	Viral Particles, Transduction	c-MYC	Human lung cancer cell line (H1299)	mRNA & Protein	Steven B. McMahon, Ph.D. The Wistar Institute
Demonstration of 3' UTR Utility, Knockdown of Overexpressed Protein	Plasmid, Transfection	FBXO6	COS-7	Protein	Kevin Glenn, M.D. Carver College of Medicine, University of Iowa
Efficiency of MISSION TurboGFP™ Transduction Control	Viral Particles, Transduction	N/A	Primary BJ Fibroblasts	Protein (FACS)	Sheila Stewart, Ph.D. Washington University
Interferon Response	Viral Particles, Transduction	CSK, AUR/KB, TYK2, YES1, AKT3	HT29	mRNA	Cell, 124, pp. 1283-1298. 2006¹
Stability of pLKO.1-puro	Plasmid DNA	N/A	N/A	Restriction Digest	Cell, 124, pp. 1283-1298. 2006¹
Dose-Response	Viral Particles, Transduction	HCK, PDGFRB, TIE1, BCR, YES1, MAP2K4, PTK7, KIS, PRKACB, MAK	HT29	Cell Viability, Mitotic Index	Cell, 124, pp. 1283-1298. 2006¹
Efficacy in Cell Types	Viral Particles, Transduction	N/A	See Table	N/A	Compilation
High-Throughput Gene Target Validation	Viral Particles, Transduction	60 Different Targets	A549	mRNA	Sigma-Aldrich R&D
High-Throughput Screening	Viral Particles, Transduction	Human Tumor Suppressor Gene Family Set	A549	Cell Viability Assay	Sigma-Aldrich R&D
High-Throughput Lentiviral Particle Production	Viral Particles	6000+ individual clones	N/A	p24 Assay	Sigma-Aldrich Operations

Publications

Duan, Z. et al Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresitance in human ovarian cancer. Mol. Cancer. Ther. 7, e-publication, 2008. Abstract

Ji, D. et al A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity. Oncol. Rep. 18, 1499-1505, 2007. Abstract

Moffat, J. et al A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell 124, 1283-1298, 2006.

For publications citing the MISSION^® TRC clones, visit the RNAi library.

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