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Sigma-Aldrich® is committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mailing MISSIONRNAi@sial.com.
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Posters – MISSION R&D
Posters – External Researchers
Selected Data Figures
Publications Citing MISSION TRC Clones
Posters – MISSION R&D
Posters – External Researchers
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Selected Data Figures
| Data type |
Format |
Gene Targets |
Cell Lines |
Detection Level |
Provided by |
| Lentivirus is stable after 2.5 years |
Viral Particles |
N/A |
A549 |
Phenotypic |
Sigma-Aldrich Operations |
| Long Term, up to 1 yr, Gene Silencing with shRNA |
Viral Particles |
ARGHDIA |
A549 |
mRNA |
Sigma-Aldrich R&D |
| Gene Target Validation |
Viral Particles, Transduction |
CDH1 |
Human colorectal cancer cell line (coll5) |
Protein |
Elena Sancho, PhD and Eduard Batlle, PhD
Barcelona Institute for Research in Biomedicine |
| Stable Cell Line/ Signaling Pathway |
Viral Particles, Transduction |
ZFP36 (TTP) |
A549 |
mRNA & Protein |
Kathleen Smoak, PhD and John A Cidlowski, PhD
Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health |
| Efficacy, Specificity and Efficiency |
Viral Particles, Transduction |
p38α, p38γ, JNK2, MAP3K4, JAK1, AKT1, AKT2, AKT3 |
Primary human astrocytes, A549, HepG2, HCT116, Panc1, HeLa |
mRNA |
Sigma-Aldrich R&D |
| Primary Astrocyte Cells (Neuronal Cells) |
Viral Particles, Transduction |
p38α, p38γ, JNK2, MAP3K4 |
Primary human astrocytes |
mRNA |
Sigma-Aldrich R&D |
| Specificity in Targeting Isoforms |
Viral Particles, Transduction |
AKT1, AKT2, AKT3 |
A549 |
mRNA |
Sigma-Aldrich R&D |
| Gene Target Validation |
Viral Particles, Transduction |
c-MYC |
Human lung cancer cell line (H1299) |
mRNA & Protein |
Steven B. McMahon, Ph.D.
The Wistar Institute |
| Demonstration of 3' UTR Utility, Knockdown of Overexpressed Protein |
Plasmid, Transfection |
FBXO6 |
COS-7 |
Protein |
Kevin Glenn, M.D.
Carver College of Medicine, University of Iowa |
| Efficiency of MISSION TurboGFP™ Transduction Control |
Viral Particles, Transduction |
N/A |
Primary BJ Fibroblasts |
Protein (FACS) |
Sheila Stewart, Ph.D.
Washington University |
| Interferon Response |
Viral Particles, Transduction |
CSK, AUR/KB, TYK2, YES1, AKT3 |
HT29 |
mRNA |
Cell, 124, pp. 1283-1298. 20061 |
| Stability of pLKO.1-puro |
Plasmid DNA |
N/A |
N/A |
Restriction Digest |
Cell, 124, pp. 1283-1298. 20061 |
| Dose-Response |
Viral Particles, Transduction |
HCK, PDGFRB, TIE1, BCR, YES1, MAP2K4, PTK7, KIS, PRKACB, MAK |
HT29 |
Cell Viability, Mitotic Index |
Cell, 124, pp. 1283-1298. 20061 |
| Efficacy in Cell Types |
Viral Particles, Transduction |
N/A |
See Table |
N/A |
Compilation |
| High-Throughput Gene Target Validation |
Viral Particles, Transduction |
60 Different Targets |
A549 |
mRNA |
Sigma-Aldrich R&D |
| High-Throughput Screening |
Viral Particles, Transduction |
Human Tumor Suppressor Gene Family Set |
A549 |
Cell Viability Assay |
Sigma-Aldrich R&D |
| High-Throughput Lentiviral Particle Production |
Viral Particles |
6000+ individual clones |
N/A |
p24 Assay |
Sigma-Aldrich Operations |
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Publications
Duan, Z., et al., Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresitance in human ovarian cancer. Mol. Cancer. Ther. 7, e-publication, 2008. Abstract
Ji, D., et al,. A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity. Oncol. Rep. 18, 1499-1505, 2007. Abstract
Moffat, J., et al., A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell 124, 1283-1298, (2006) Abstract
For publications citing the MISSION® TRC clones, visit the RNAi library.
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Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.
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