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MISSION® Application Data

MISSION shRNA

Sigma-Aldrich® is committed to providing you with helpful application notes and data for the MISSION® TRC shRNA libraries. Below are links to data pieces and articles on various topics of interest. If you would like us to post your data (specific applications, validation of gene targets, etc.) on our site, please send us your experimental summary and data by e-mailing MISSIONRNAi@sial.com.


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Posters – MISSION R&D
Posters – External Researchers
Selected Data Figures
Publications Citing MISSION TRC Clones


Posters – MISSION R&D

  • A Screen of MISSION shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in A549 Paclitaxel Sensitivity
  • Gene Silencing in Primary Astrocyte Cells using MISSION TRC shRNA Lentiviral Particles (338 Kb PDF)
  • Reproducible, Long-term Silencing in a High-throughput Lentiviral shRNA Screen (211 Kb PDF)
  • Verification of RNA Interference at the Protein Level via Quantitative Proteomics Analysis (374 Kb PDF)
  • Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library (357 Kb PDF)
  • Efficient Gene Silencing with High Selectivity using MISSION TRC shRNA Lentiviral Vectors (199 Kb PDF)

Posters – External Researchers

  • Lentiviral shRNA Screen of Multidrug Resistant Associated Genes Identifies PRP-4 as a New Regulator of Chemoresistance in Human Ovarian Cancer (635 Kb PDF)
    Zhenfeng Duan, M.D. Ph.D. – Harvard Medical School

  • shRNA Phenotypic Screen To Identify Gene Targets Contributing to Androgen Independent Prostate Cancer Cell Growth (989 Kb PDF)
    Daniel Gioeli Ph.D. – University of Virginia

  • Mesenchymal Stem Cells: Cell Death or Survival? Interaction with Other Stress Pathways (887 Kb PDF)
    by Oliver L. and Vallette FM, Center for Cancer Research Nantes - Angers INSERM UMR 892, University of Nantes, France

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Selected Data Figures

Data type Format Gene Targets Cell Lines Detection Level Provided by
Lentivirus is stable after 2.5 years Viral Particles N/A A549 Phenotypic Sigma-Aldrich Operations
Long Term, up to 1 yr, Gene Silencing with shRNA Viral Particles ARGHDIA A549 mRNA Sigma-Aldrich R&D
Gene Target Validation Viral Particles, Transduction CDH1 Human colorectal cancer cell line (coll5) Protein Elena Sancho, PhD and Eduard Batlle, PhD
Barcelona Institute for Research in Biomedicine
Stable Cell Line/ Signaling Pathway Viral Particles, Transduction ZFP36 (TTP) A549 mRNA & Protein Kathleen Smoak, PhD and John A Cidlowski, PhD
Laboratory of Signal Transduction, National Institute of Environmental Health Services, National Institutes of Health
Efficacy, Specificity and Efficiency Viral Particles, Transduction p38α, p38γ, JNK2, MAP3K4, JAK1, AKT1, AKT2, AKT3 Primary human astrocytes, A549, HepG2, HCT116, Panc1, HeLa mRNA Sigma-Aldrich R&D
Primary Astrocyte Cells (Neuronal Cells) Viral Particles, Transduction p38α, p38γ, JNK2, MAP3K4 Primary human astrocytes mRNA Sigma-Aldrich R&D
Specificity in Targeting Isoforms Viral Particles, Transduction AKT1, AKT2, AKT3 A549 mRNA Sigma-Aldrich R&D
Gene Target Validation Viral Particles, Transduction c-MYC Human lung cancer cell line (H1299) mRNA & Protein Steven B. McMahon, Ph.D.
The Wistar Institute
Demonstration of 3' UTR Utility, Knockdown of Overexpressed Protein Plasmid, Transfection FBXO6 COS-7 Protein Kevin Glenn, M.D.
Carver College of Medicine, University of Iowa
Efficiency of MISSION TurboGFP™ Transduction Control Viral Particles, Transduction N/A Primary BJ Fibroblasts Protein (FACS) Sheila Stewart, Ph.D.
Washington University
Interferon Response Viral Particles, Transduction CSK, AUR/KB, TYK2, YES1, AKT3 HT29 mRNA Cell, 124, pp. 1283-1298. 20061
Stability of pLKO.1-puro Plasmid DNA N/A N/A Restriction Digest Cell, 124, pp. 1283-1298. 20061
Dose-Response Viral Particles, Transduction HCK, PDGFRB, TIE1, BCR, YES1, MAP2K4, PTK7, KIS, PRKACB, MAK HT29 Cell Viability, Mitotic Index Cell, 124, pp. 1283-1298. 20061
Efficacy in Cell Types Viral Particles, Transduction N/A See Table N/A Compilation
High-Throughput Gene Target Validation Viral Particles, Transduction 60 Different Targets A549 mRNA Sigma-Aldrich R&D
High-Throughput Screening Viral Particles, Transduction Human Tumor Suppressor Gene Family Set A549 Cell Viability Assay Sigma-Aldrich R&D
High-Throughput Lentiviral Particle Production Viral Particles 6000+ individual clones N/A p24 Assay Sigma-Aldrich Operations


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Publications

Duan, Z., et al., Lentiviral short hairpin RNA screen of genes associated with multidrug resistance identifies PRP-4 as a new regulator of chemoresitance in human ovarian cancer. Mol. Cancer. Ther. 7, e-publication, 2008. Abstract

Ji, D., et al,. A screen of shRNAs targeting tumor suppressor genes to identify factors involved in A549 paclitaxel sensitivity. Oncol. Rep. 18, 1499-1505, 2007. Abstract

Moffat, J., et al., A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell 124, 1283-1298, (2006) Abstract

For publications citing the MISSION® TRC clones, visit the RNAi library.

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Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.



MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.

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