pLKO 1 Stability

pLKO.1 Stability Assessed by Diagnostic Restriction Digestion

Digestion of pLKO.1 library constructs by PvuII produces three fragments of 3.8 kb, 2.5 kb, and 0.8 kb. PvuII digestions of 244 library clones in pLKO.1 were performed following ten rounds of sequential copying and regrowth. No evidence of recombination was observed. A representative digestion gel from a fraction of wells in a library plate from a 10^th generation copy is shown.

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