Can plasmid shRNA deliver stable gene silencing?
Why would I use the purified plasmid DNA format?
Can plasmid shRNA deliver stable gene silencing?
The efficiency of transduction of lentivirus is much higher for stable expression, but plasmid-based transfections will also work. That said, in the specific case of the liver, stable transduction of a small population can be achieved through hydrodynamic delivery of plasmid DNA.1
- Liu, F., Song, Y. & Liu, D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther 6, 1258-1266 (1999). Pubmed ID 10455434
Why would I use the purified plasmid DNA format?
The DNA format (1 µg) is ideal for doing small transfections in 96-well plates (with replicates) or for small-scale packaging co-tranfections. Plasmid purification is a costly and time-consuming step that is eliminated with this product format.
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