Troubleshooting

What should I do if I have trouble obtaining high yields of plasmid DNA?
Generally, high yields of DNA can be achieved with much of the collection; however, viral-based vectors are known to be somewhat difficult to prep. If particular clones are troublesome, we recommend streaking the bacterial stocks on LB/carbenicillin plates to isolate a single colony and DNA purification with GenElute™ Endotoxin free Midi (Product Code NA0200) or GenElute™ Endotoxin free HP Maxi (Product Code NA0400S) prep kits.

Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.

MISSION is a trademark of Sigma-Aldrich. Label License.

back to top
RNAi FAQ Center
shRNA Homepage
RNAi Homepage