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Introducing TRC1 and TRC2: Start your shRNA Research Today!
The RNAi Consortium (TRC) is a collaborative group of world-renowned academic and corporate life science research groups whose "mission" is to create comprehensive tools for functional genomics research and make them broadly available to scientists worldwide. TRC1 contains 150,000+ pre-cloned shRNA constructs. TRC2 also contains 150,000+ pre-cloned shRNA constructs.
Sigma-Aldrich is both a collaborative member and supplier of TRC1 and TRC2, the most comprehensive and thoroughly validated shRNA collection available.
MISSION shRNA Library
The TRC1 human and mouse shRNA libraries were developed at the Broad Institute of MIT and Harvard, and currently consists of 159,000 pre-cloned shRNA constructs targeting 16,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,950 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. Sigma is the exclusive manufacturer of TRC1 clones in DNA and lentiviral formats. Glycerol format products are also available.
 Comprehensive shRNA Collection
Optimal Lentiviral-Based RNAi
For introductory information on working with shRNA and /or Lentivirus, visit: MISSION shRNA Library
The TRC1 human and mouse shRNA libraries were developed at the Broad Institute of MIT and Harvard, and currently consists of 159,000 pre-cloned shRNA constructs targeting 16,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,950 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. Sigma is the exclusive manufacturer of TRC1 clones in DNA and lentiviral formats. Glycerol format products are also available.
Comprehensive shRNA Collection
Optimal Lentiviral-Based RNAi
For introductory information on working with shRNA and /or Lentivirus, visit: Membership in TRC2 and Validation Efforts
Sigma-Aldrich is a member and current sole distributor of TRC2. Current plans for TRC2 include functional testing and validation of the TRC1 library, creation of an additional 150,000 TRC2 clones (total of 300,000), and functional testing of the new TRC2 clones, while looking at improved methods for screening. TRC1 validation efforts are already underway. Combined efforts of The Broad Institute and Sigma-Aldrich have generated validation data for over 4500 TRC1 gene targets that are now designated as “Validated” on the shRNA detail pages. Validation requires that 1 or more shRNAs have been functionally tested and have demonstrated 70% or greater knockdown levels. Search Your Favorite Gene Search to locate shRNAs that target your gene. Look for the validated symbol located on the shRNA detail pages to identify functionally validated shRNAs.
MISSION shRNA Design
MISSION shRNA clones, designed and developed by TRC at the Broad Institute of MIT and Harvard, include hairpin sequences comprised of a 21 base stem and a 6 base loop. Rules based design consisting of sequence, specificity, and position scoring are utilized to generate optimal shRNA sequences. The hairpin sequences are each cloned into the pLKO.1 vector and sequence verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. One in five clones will typically provide at least 70% knockdown of the gene target. For a given RefSeq, many shRNA clone sets include a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs.
shRNA Designed for High Performance
 Click here to view enlarged image (259 Kb PDF) Figure 1. High-throughput validation of MISSION-TRC shRNA lentiviral transduction particle. Gene silencing effect of 60 different genes was measured at the mRNA transcript level and compared to pLKO.1-puro empty control particles. For each target gene, approximately 70% or greater knockdown was acheived.
Lentiviral Production Expertise
Sigma-Aldrich is committed to providing unmatched infrastructure and service for RNAi. With the addition of state-of-the-art robotics, liquid handling, LIMS, and dedicated laboratories in our new Life Science & High Technology Center, our goal is to provide superior quality and convenient service for your RNAi research needs.
High Quality Manufacturing Systems and Personnel
The Leader in Lentiviral shRNA Products and Support
Screening Formats for Any Scale
Sigma-Aldrich is committed to providing you powerful and unique screening formats that fully leverage the discovery potential of the trusted and proven TRC shRNA collection. When you partner with Sigma Life Sciences you gain access to our world class lentiviral production expertise and the formats you require for systematic or pooled RNAi screening on any scale
Interested in genome-wide discovery work? LentiElite, the entire pre-arrayed human or mouse library, is available in either 96- or 384-well formats. For rapid, whole-genome pooled shRNA screens, LentiPlex pooled libraries are the answer. Pooled screening presents the entire library in ten vials, and affords expeditious phenotypic, positive selection screens. If you’re more interested in focused screening relating to a specific gene family, pathway, process, function or disease relating to your biological question, we offer a host of pre-designed gene family sets and panels to make your screening easier. If you are looking for a quick Human Kinase screening tool, LentiExpress, a reverse transduction method is available. Do you know exactly the gene you need? Explore YFG to find individual clones for your gene of interest!
From whole genome libraries to individual clones, the MISSION TRC shRNA Lentiviral collection is your path to unlimited discovery. Wide Array of Product Formats
Flexible Custom Capabilities
Sigma-Aldrich is the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format. With world-class lentiviral manufacturing expertise and industry leading custom flexibility, Sigma provides everything you need for even the most unique RNAi project. Additionally, a number of customizable options (including high-throughput projects, custom cloning and large-scale/high titer lentivirus) are available at your fingertips with are new improved ordering interface.
All custom requests will be considered.
 Rarely, one size fits all. Small, medium or large — your research needs are your own. So, our technology comes custom-fit with your basic and applied research in mind.
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