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Introducing TRC1 and TRC2: Start Your shRNA Research Today! The RNAi Consortium (TRC) is a collaborative group of world-renowned academic and corporate life science research groups whose "mission" is to create comprehensive tools for functional genomics research and make them broadly available to scientists worldwide. TRC1 contains more than 159,000 pre-cloned shRNA constructs. In addition, Sigma® is also the exclusive supplier of the TRC1.5 library, which is additional clone content of more than 18,000 pre-cloned shRNA constructs in the same vector backbone as TRC1. TRC2 is currently being made and will contain approximately 150,000 pre-cloned shRNA constructs. Sigma-Aldrich® is both a collaborative member and supplier of TRC1 and TRC2, the most comprehensive and thoroughly validated shRNA collection available.
MISSION shRNA Library The TRC1 human and mouse shRNA libraries were developed at the Broad Institute of MIT and Harvard, and currently consists of more than 159,000 pre-cloned shRNA constructs targeting more than 16,000 annotated human genes (MISSION TRC-Hs 1.0) and more than 15,950 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. Sigma is the exclusive manufacturer of TRC1 clones in DNA and lentiviral formats. Glycerol format products are also available.
Optimal Lentiviral-Based RNAi
For introductory information on working with shRNA and/or Lentivirus, visit: Getting Started with shRNA Membership in TRC2 and Validation Efforts Sigma-Aldrich is a member and current sole distributor of TRC2. Current plans for TRC2 include functional testing and validation of a portion of the TRC1 library, creation of an additional 150,000 TRC2 clones (total of approximately 300,000), and functional testing of the new TRC2 clones, while looking at improved methods for screening. TRC1 validation efforts are already underway. Combined efforts of The Broad Institute and Sigma-Aldrich have generated validation data for over 38,000 clones and 11,000 gene targets that are now designated as "Validated" on the shRNA clones detail pages. Validation requires that 1 or more shRNA has been functionally tested and has demonstrated 50% or greater knockdown levels. Search Your Favorite Gene to locate shRNAs that target your gene. Look for the validated symbol on the shRNA clones detail pages to identify functionally validated shRNAs. Validation is Currently Underway!
MISSION shRNA Design MISSION shRNA clones, designed and developed by TRC at the Broad Institute of MIT and Harvard, include hairpin sequences comprised of a 21 base stem and a 6 base loop. Rules based design consisting of sequence, specificity, and position scoring are utilized to generate optimal shRNA sequences. The hairpin sequences are each cloned into the pLKO.1 vector and sequence verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. Sigma-Aldrich guarantees that upon purchase of the defined clone set quantity on the shRNA detail page for your gene of interest (this varies, but is typically 5 clones), that at least one of those clones for a gene should yield greater than 70% knockdown. For a given RefSeq, there is often a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs. shRNA Designed for High Performance
Lentiviral Production Expertise Sigma-Aldrich is committed to providing unmatched infrastructure and service for RNAi. With the addition of state-of-the-art robotics, liquid handling, LIMS, and dedicated laboratories in our new Life Science & High Technology Center, our goal is to provide superior quality and convenient service for your RNAi research needs. High Quality Manufacturing Systems and Personnel
The Leader in Lentiviral shRNA Products and Support
Screening Formats for Any Scale Sigma-Aldrich is committed to providing you powerful and unique screening formats that fully leverage the discovery potential of the trusted and proven TRC shRNA collection. When you partner with Sigma Life Sciences, you gain access to our world class lentiviral production expertise and the formats you require for systematic or pooled RNAi screening on any scale
Interested in genome-wide discovery work? LentiElite™, the entire pre-arrayed human or mouse library, is available in either 96- or 384-well formats. For rapid, whole-genome pooled shRNA screens, LentiPlex™ pooled libraries are the answer. Pooled screening presents the entire library in ten vials, and affords expeditious phenotypic, positive selection screens. If you're more interested in focused screening relating to a specific gene family, pathway, process, function or disease relating to your biological question, we offer a host of pre-designed gene family sets and panels to make your screening easier. If you are looking for a quick Human Kinase screening tool, LentiExpress™, a reverse transduction method is available. Do you know exactly the gene you need? Explore YFG to find individual clones for your gene of interest! Wide Array of Product Formats
Flexible Custom Capabilities Sigma-Aldrich is the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format. With world-class lentiviral manufacturing expertise and industry leading custom flexibility, Sigma provides everything you need for even the most unique RNAi project. Additionally, a number of customizable options (including high-throughput projects, custom cloning and large-scale/high titer lentivirus) are available at your fingertips with our new improved ordering interface.
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