Introducing the New MISSION shRNA Library, powered by TRC.
Do you want to perform RNAi in non-transfectable or non-dividing cells? Do you need efficient, long-term knockdown and require a robust, quality system? Sigma® Life Science and The RNAi Consortium (TRC) provide the MISSION shRNA Library for human and mouse genomes, developed at the Broad Institute, a joint venture between MIT and Harvard.
The MISSION shRNA Library is exclusive to Sigma Life Science and contains almost 250,000 clones including more than 80,000 validated clones. It combines all of the highly trusted TRC1 content plus an additional 88,000 clones targeting 4,000 new human genes and more than 5,000 new mouse genes!
Sigma-Aldrich is both a collaborative member and supplier of the MISSION shRNA Library, the most comprehensive and thoroughly validated shRNA collection available.
Start Your shRNA Research Today
Why experiment witht the old TRC library when you can get the bigger and better MISSION shRNA Library? The MISSION shRNA Library is exclusive to Sigma Life Science and contains almost 250,000 clones including more than 80,000 validated clones. It combines all of the highly trusted TRC1 content plus an additional 88,000 clones targeting 4,000 new human genes and more than 5,000 new mouse genes: The new MISSION shRNA library provides unparalleled genome coverage and is available only at Sigma Life Science. Start your research today with the largest and most comprehensive genome-wide shRNA library on the market!
Optimal Lentiviral-Based RNAi
For introductory information on working with shRNA and/or Lentivirus, visit: Getting Started with shRNA
| MISSION shRNA Library vs. TRC1 Library | ||||
| Total | Validated | Total | Validated | |
| Human Genes | 20,018 | 8,897 | 16,019 | 7,405 |
| Mouse Genes | 21,171 | 8,345 | 15,960 | 6,506 |
| Total Genes | 41,189 | 17,242 | 31,979 | 13,911 |
| Human Clones | 129,695 | 43,470 | 80,717 | 24,550 |
| Mouse Clones | 118,062 | 36,654 | 77,819 | 20,564 |
| Total Clones | 247,757 | 80,124 | 159,836 | 45,114 |
| Final clone counts may vary, as the library is constantly updated for accuracy of annotation and content. | ||||
MISSION shRNA Design
MISSION shRNA clones, designed and developed by TRC at the Broad Institute of MIT and Harvard, include hairpin sequences comprised of a 21 base stem and a 6 base loop. Rules based design consisting of sequence, specificity, and position scoring are utilized to generate optimal shRNA sequences. The hairpin sequences are each cloned into the pLKO.1 vector and sequence verified. A minimum of 3-5 shRNA constructs are created for each target gene to provide varying levels of knockdown and to target different regions of mRNA transcript. Sigma-Aldrich guarantees that upon purchase of the defined clone set quantity on the shRNA detail page for your gene of interest (this varies, but is typically 5 clones), that at least one of those clones for a gene should yield greater than 70% knockdown. For a given RefSeq, there is often a shRNA clone targeting the 3'UTR for use in phenotypic rescue studies using cDNA expression constructs.
shRNA Designed for High Performance
Click on image to enlarge. (281 Kb PDF)
| Figure 1. High-throughput validation of MISSION-TRC shRNA lentiviral transduction particles. Gene silencing effect of 60 different genes was measured at the mRNA transcript level and compared to pLKO.1-puro empty control particles. For each target gene, approximately 70% or greater knockdown was achieved. |
Lentiviral Production Expertise
Sigma-Aldrich is committed to providing unmatched infrastructure and service for RNAi. With the addition of state-of-the-art robotics, liquid handling, LIMS, and dedicated laboratories in our new Life Science & High Technology Center, our goal is to provide superior quality and convenient service for your RNAi research needs.
High Quality Manufacturing Systems and Personnel
The Leader in Lentiviral shRNA Products and Support
Screening Formats for Any Scale
Sigma-Aldrich is committed to providing you powerful and unique screening formats that fully leverage the discovery potential of the trusted and proven TRC shRNA collection. When you partner with Sigma Life Sciences, you gain access to our world class lentiviral production expertise and the formats you require for systematic or pooled RNAi screening on any scale
For rapid, whole-genome pooled shRNA screens, LentiPlex® pooled libraries are the answer. Pooled screening presents the entire library in ten vials, and affords expeditious phenotypic, positive selection screens. If you're more interested in focused screening relating to a specific gene family, pathway, process, function or disease relating to your biological question, we offer a host of pre-designed gene family sets and panels to make your screening easier. If you are looking for a quick Human Kinase screening tool, LentiExpress™, a reverse transduction method is available. Do you know exactly the gene you need? Explore YFG to find individual clones for your gene of interest!
From whole genome libraries to individual clones, the MISSION TRC shRNA Lentiviral collection is your path to unlimited discovery.
Wide Array of Product Formats
Flexible Custom Capabilities
Sigma-Aldrich is the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format. With world-class lentiviral manufacturing expertise and industry leading custom flexibility, Sigma provides everything you need for even the most unique RNAi project. Additionally, a number of customizable options (including high-throughput projects, custom cloning and large-scale/high titer lentivirus) are available at your fingertips with our new improved ordering interface.
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All custom requests will be considered. Rarely, one size fits all. Small, medium or large — your research needs are your own. So, our technology comes custom-fit with your basic and applied research in mind. |