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shRNA
Whole-Genome shRNA Libraries
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Partner with Sigma for your genome-wide shRNA screening. Sigma is the exclusive provider of TRC clones in purified DNA and lentiviral formats for any combination of TRC clones. Bacterial glycerol stock format is also available. When deciding upon the most appropriate biologic format to begin with, we suggest MISSION LentiElite™ Arrayed Libraries. LentiElite is a ready-to-go lentivirus screen leveraging the entire collection of 150,000 TRC shRNA clones for either human or mouse whole genome screening. A single shRNA is individually arrayed per well in either a 96- or 384-well format. On average, there are five separate shRNAs targeting the same gene.
MISSION LentiElite™ Arrayed Libraries
- Immediate – Start screening today without spending months to years on scale-up and viral production
- Superior Quality – Screen with confidence when you rely on the trusted TRC shRNA library
- Accessible – Minimize investments in robotics and dedicated personnel for lentiviral manufacturing
| Product Description |
Product Number |
| MISSION LentiElite Human Library |
LIBHV1 |
| MISSION LentiElite Mouse Library |
LIBMV1 |
The TRC1 shRNA collection, developed at the Broad Institute of MIT and Harvard, includes over 150,000 pre-cloned shRNA constructs targeting greater than 15,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,000 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. In addition, we are striving to functionally validate every shRNA within the TRC collection, and providng that data back to you. Sigma-Aldrich offers a variety of products to support the downstream validation of your shRNA results. These products include siRNA, antibodies, small molecules, purified peptides and proteins (see Table 1). To specifically see what products we have for your gene of interest, utilize the Your Favorite Gene™ search engine.
Please inquire at RNAi@sial.com, or speak to your local Sigma sales representative, to receive additional information or quotes for whole-genome shRNA libraries. Please specify human, mouse or both. Also indicate your preferred biologic format (lentiviral, purified plasmid DNA or bacterial glycerol stock), and well format (96- or 384-well).
More shRNA Screening Solutions
Table 1. Supporting Products
The table summarizes examples of supporting gene-based products for shRNA Libraries. For additional related products, please visit the RNAi Workflow.
| Number of Supporting Products |
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Human |
Mouse |
| siRNAs |
371679 |
201514 |
| Antibodies |
10060 |
1722 |
| Small Molecules |
4887 |
437 |
| AQUA Peptides |
484 |
236 |
| Peptides and Proteins |
713 |
88 |
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Table 2. Current Library by Gene Family or Functional Class
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The gene families indicated below are a sample of the content found in the collection and are not inclusive of the genes found in the libraries. If you are interested in a specific gene family, please inquire at RNAi@sial.com You may also send us your gene lists for us to query the collection. For pre-arrayed gene family sets, please visit the shRNA Gene Families page or search using the Your Favorite Gene™ search engine for your targets of interest.
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Gene Family/
Functional Class |
| Amidase |
| Apoptosis |
| Carboxylase |
| Cyclase |
| Cytokine and Chemokine Receptors |
| Cytokines and Chemokines |
| DNA Binding |
| DNA Repair |
| Deacetylase |
| Dehydrogenase |
| Electron Transport |
| Esterase |
| G Protein |
| GPCR |
| GTPase |
| Glucosidase |
| Homeobox |
| Hydrolase |
| Ion Channel |
| Isomerase |
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Gene Family/
Functional Class |
| Kinase |
| Ligase |
| Lipase |
| Methylase |
| Mutase |
| Nuclear Hormone Receptor |
| Nuclease |
| Oxidase |
| Oxidoreductase |
| Phosphatase |
| Polymerase |
| Protease |
| Protein Transport |
| Reductase |
| Spliceosome |
| Synthase |
| Transcription Factor |
| Transferase |
| Tumor Suppressor |
| Tyrosine Kinase |
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