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Life Science > Functional Genomics & RNAi > shRNA > Library Information > Whole-Genome shRNA Libraries
shRNA

Whole-Genome shRNA Libraries


MISSION shRNA

Partner with Sigma for your genome-wide shRNA screening. Sigma is the exclusive provider of TRC clones in purified DNA and lentiviral formats for any combination of TRC clones. Bacterial glycerol stock format is also available. When deciding upon the most appropriate biologic format to begin with, we suggest MISSION LentiElite™ Arrayed Libraries. LentiElite is a ready-to-go lentivirus screen leveraging the entire collection of 150,000 TRC shRNA clones for either human or mouse whole genome screening. A single shRNA is individually arrayed per well in either a 96- or 384-well format. On average, there are five separate shRNAs targeting the same gene.

MISSION LentiElite™ Arrayed Libraries

  • Immediate – Start screening today without spending months to years on scale-up and viral production
  • Superior Quality – Screen with confidence when you rely on the trusted TRC shRNA library
  • Accessible – Minimize investments in robotics and dedicated personnel for lentiviral manufacturing

Product Description Product Number
MISSION LentiElite Human Library LIBHV1
MISSION LentiElite Mouse Library LIBMV1

The TRC1 shRNA collection, developed at the Broad Institute of MIT and Harvard, includes over 150,000 pre-cloned shRNA constructs targeting greater than 15,000 annotated human genes (MISSION TRC-Hs 1.0) and 15,000 annotated mouse genes (MISSION TRC-Mm 1.0) for RNA interference-mediated gene silencing. In addition, we are striving to functionally validate every shRNA within the TRC collection, and providng that data back to you. Sigma-Aldrich offers a variety of products to support the downstream validation of your shRNA results. These products include siRNA, antibodies, small molecules, purified peptides and proteins (see Table 1). To specifically see what products we have for your gene of interest, utilize the Your Favorite Gene™ search engine.

Please inquire at RNAi@sial.com, or speak to your local Sigma sales representative, to receive additional information or quotes for whole-genome shRNA libraries.  Please specify human, mouse or both.  Also indicate your preferred biologic format (lentiviral, purified plasmid DNA or bacterial glycerol stock), and well format (96- or 384-well).

More shRNA Screening Solutions

Table 1. Supporting Products
The table summarizes examples of supporting gene-based products for shRNA Libraries.  For additional related products, please visit the RNAi Workflow.

Number of Supporting Products
  Human Mouse
siRNAs 371679 201514
Antibodies 10060 1722
Small Molecules 4887 437
AQUA Peptides 484 236
Peptides and Proteins 713 88

Table 2. Current Library by Gene Family or Functional Class

The gene families indicated below are a sample of the content found in the collection and are not inclusive of the genes found in the libraries.  If you are interested in a specific gene family, please inquire at RNAi@sial.com  You may also send us your gene lists for us to query the collection.  For pre-arrayed gene family sets, please visit the shRNA Gene Families page or search using the Your Favorite Gene™ search engine for your targets of interest.

Gene Family/
Functional Class
Amidase
Apoptosis
Carboxylase
Cyclase
Cytokine and Chemokine Receptors
Cytokines and Chemokines
DNA Binding
DNA Repair
Deacetylase
Dehydrogenase
Electron Transport
Esterase
G Protein
GPCR
GTPase
Glucosidase
Homeobox
Hydrolase
Ion Channel
Isomerase
 
Gene Family/
Functional Class
Kinase
Ligase
Lipase
Methylase
Mutase
Nuclear Hormone Receptor
Nuclease
Oxidase
Oxidoreductase
Phosphatase
Polymerase
Protease
Protein Transport
Reductase
Spliceosome
Synthase
Transcription Factor
Transferase
Tumor Suppressor
Tyrosine Kinase

Contact Us

For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.



MISSION is a trademark of Sigma-Aldrich. Label License.

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