shRNA

MISSION Custom Request

Trust in MISSION® Custom Services to create the perfect clone to fit your research. With access to 14 customizable vectors, a wide range of titer and volume options, and of course, functionally validated shRNA sequences, be assured that our dedicated team can generate the right clone for you.

Sigma® offers a number of customizable solutions for the MISSION TRC and Lentiviral based manufacturing platform. Please use this request form to submit information to receive a quotation. A representative from Sigma-Aldrich will provide a quotation number to you within two business days of your submission or contact you for additional information.

Large Scale and High Titer Lentiviral Manufacturing
Custom Cloning
Other Services

Large Scale and High Titer Lentiviral Particles
The pricing below is for individual clones and controls of the TRC collection. Please determine the option that best suits your needs and proceed to the custom request submission form at the bottom of the page. For all other scales, titers, or personal clones, please request the "Custom-8" option. Manufacturing time is approximately 4-7 weeks depending upon the clone and scale ordered.

Our Standard Packaging Configurations

Option Titer Volume Packaging Min. # Particles
Custom-1 1 x 106 TU/ml* 1 ml 5 x 200 µl 1 x 106
Custom-2 1 x 106 TU/ml 5 ml 25 x 200 µl 5 x 106
Custom-3 1 x 108 TU/ml 0.2 ml 4 x 50 µl 20 x 106
Custom-4 1 x 108 TU/ml 1 ml 10 x 100 µl 100 x 106
Custom-5 1 x 108 TU/ml 2 ml 20 x 100 µl 200 x 106
Custom-6 1 x 109 TU/ml 0.2 ml 4 x 50 µl 200 x 106
Custom-7 1 x 109 TU/ml 1 ml 10 x 100 µl 1000 x 106
Custom-8 Other Other Other Other

* Standard minimum titer is 1 x 106 TU/ml via p24 assay (yields are on average >1 x 107 TU/ml via p24 assay).
If you require a specific titer, please request the Custom-8 option.

Go to Request Form

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Custom Cloning
Sigma has a custom cloning team for the MISSION shRNA product group. Currently the options in the table below are available. Check back periodically as the service and options continue to expand. Manufacturing time is approximately 4–6 weeks. For more information about our custom services, please visit our custom capabilities section. For vector sequences and maps, please visit our vector sequences page. Please determine the option that best suits your needs and proceed to the custom request submission form at the bottom of the page.

Please Note: custom DNA or custom viral particles are available. For an additional charge, both may be ordered.


Too many options? Send us an e-mail at MISSIONRNAi@sial.com, and we’ll have someone contact you.
Option Vector Insert DNA Provided Virus Provided
Custom-9 pLKO.1-puro Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-10 pLKO.1-CMV-TurboGFP™ TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-11 pLKO.1-CMV-TurboGFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-12 pLKO.1-hPGK-puro-CMV-TurboGFP TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-13 pLKO.1-hPGK-puro-CMV-TurboGFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-14 pLKO.1-CMV-Neo TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-15 pLKO.1-CMV-Neo Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-16 pLKO.1-hPGK-Neo TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-17 pLKO.1-hPGK-Neo Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-18 pLKO.1-hPGK-Neo-CMV-TurboGFP TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-19 pLKO.1-hPGK-Neo-CMV-TurboGFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-20 pLKO.1-hPGK-puro-CMV-TagCFP™ TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-21 pLKO.1-hPGK-puro-CMV-TagCFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-22 pLKO.1-hPGK-puro-CMV-TagYFP™ TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-23 pLKO.1-hPGK-puro-CMV-TagYFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-24 pLKO.1-hPGK-puro-CMV-TagRFP™ TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-25 pLKO.1-hPGK-puro-CMV-TagRFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-26 pLKO.1-hPGK-puro-CMV-TagFP635™ TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-27 pLKO.1-hPGK-puro-CMV-TagFP635 Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-28 pLKO.1-hPGK-puro-UbC-TurboGFP TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-29 pLKO.1-hPGK-puro-UbC-TurboGFP Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-30 pLKO.1-hPGK-puro-UbC-TagFP635 TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-31 pLKO.1-hPGK-puro-UbC-TagFP635 Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-32 pLKO-puro-IPTG-1xLacO TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-33 pLKO-puro-IPTG-1xLacO Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-34 pLKO-puro-IPTG-3xLacO TRC shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-35 pLKO-puro-IPTG-3xLacO Customer shRNA sequences 1 µg 200 µl, 1 x 106 TU/ml
Custom-36 Other Other Other Other

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Other Services
Sigma’s highly trained and professional staff often accommodates other unique requests. Please use the comment section (question #4) of the form below to describe your request.

Request Form
Please answer the following fields and questions to receive your quotation information.