shRNA

MISSION® LentiPlex® Pooled shRNA Libraries

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Save even more on Pooled shRNA Libraries: Use Promo Code 998 to save up to 15% on human and mouse MISSION LentiPlex Complete Pooled shRNA Libraries!


For Rapid, Convenient Genome-wide shRNA Screens

MISSION LentiPlex Pooled shRNA Libraries bring the power of the MISSION TRC shRNA collection together with Sigma's lentiviral manufacturing expertise to enable genome-wide RNAi screens on your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

  • Comprehensive – Trusted TRC content with shRNA targeting 20,000+ human or 21,000+ mouse genes
  • Coverage – Average of 5–6 clones targeting each gene
  • Quality – Proprietary methodology ensures uniformity and complete library coverage
  • Universal – Simplified whole genome RNAi screening in standard or difficult to expand cell lines
  • Accessible – Bench top discovery without robotics or liquid handling system
  • Versatile – Compatible with FACS and most downstream fluorescent assays

Product Specifications

The MISSION LentiPlex Human and Mouse Pooled shRNA consist of ~130,000 (over 43,000 validated) shRNA constructs targeting 20,000+ human genes and ~118,000 (over 36,000 validated) shRNA constructs targeting 21,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage and is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay. The MISSION LentiPlex libraries are pre-divided into various subpools (see Table 1 and Table 2 below for number of subpools and approximate number of shRNA constructs represented in each). Amplification and sequencing primers are also provided for downstream target identification.

  • Pre-aliquoted into subpools ranging from 5,500 to 8,000 shRNA constructs per pool
  • Enough material for six screens at 100X representation
  • Titers of at least 5 x 108 TU/ml
  • Amplification and sequencing primers provided

Ordering Information

Product Description Product Number
MISSION LentiPlex Human Pooled shRNA Library SHPHLIBR
MISSION LentiPlex Mouse Pooled shRNA Library SHPMLIBR

Please contact your local sales representative for additional information.

 

LentiPlex Contents (Human and Mouse)


MISSION LentiPlex Pooled shRNA Human Library


Clone Coverage by Library

Product Name Product No. Approx. Total Clone Coverage No. of Genes No. of Subpools Approx. No. of Clones/subpool
LentiPlex 1.0 SHPH01 80,000+ 16,000+ 10 8000
LentiPlex 1.5 SHPH15 25,000+ 6,000+ 3 8000
LentiPlex 2.0 SHPH2 20,000+ 5,000+ 3 6000
LentiPlex Complete
(1.0 + 1.5 + 2.0)
SHPHLIBR 125,000+ 20,000+ 16 6000 or 8000

Table 1. The MISSION LentiPlex Human Pooled shRNA Library, Catalog Numbers SHPH01, SHPH15, SHPH2, SHPHLIBR are genome-wide lentiviral pools, powered by the RNAi Consortium (TRC). Each version of TRC (1.0, 1.5, and 2.0) corresponds to the LentiPlex Product Name and Number. The addition of LentiPlex 1.5 and 2.0 offer expanded gene and clone coverage from the original TRC1 library. The LentiPlex Complete consists of the full shRNA library of over 125,000 clones and targeting over 20,000 human genes.



MISSION LentiPlex Pooled shRNA Mouse Library


Clone Coverage by Library

Product Name Product No. Approx. Total Clone Coverage No. of Genes No. of Subpools Approx. No. of Clones/subpool
LentiPlex 1.0 SHPM01 75,000+ 15,000+ 10 8,000
LentiPlex 1.5 SHPM15 10,000+ 4,000+ 2 5,500
LentiPlex 2.0 SHPM2 25,000+ 7,000+ 3 8,000
LentiPlex Complete
(1.0 + 1.5 + 2.0)
SHPMLIBR 110,000+ 21,000+ 15 5,500 or 8,000

Table 2. The MISSION LentiPlex Mouse Pooled shRNA Library, Catalog Numbers SHPM01, SHPM15, SHPM2, SHPMLIBR are genome-wide lentiviral pools, powered by the RNAi Consortium (TRC). Each version of TRC (1.0, 1.5, and 2.0) corresponds to the LentiPlex Product Name and Number. The addition of LentiPlex 1.5 and 2.0 offer expanded gene and clone coverage from the original TRC1 library. The LentiPlex Complete consists of the full shRNA library of over 110,000 clones and targeting over 21,000 mouse genes. 


Highly Validated Gene and Clone Contents

  Total Validated
Human Clones 129,000+ 43,000+
Mouse Clones 118,000+ 36,000+
Total Clones 247,000+ 80,000+
Human Genes
20,000+ 8,000+
Mouse Genes
21,000+ 8,000+
Total Genes
41,000+ 16,000+


MISSION custom pooled viral shRNA libraries

Pooled shRNA RNAi screening approaches are fast becoming an economical alternative for cell based screens. MISSION RNAi is now offering custom pooled shRNA viral libraries. Just give us your list of genes or pick a gene set and leave the rest to us!

Contact your local Sigma sales representative for more information or submit an inquiry to MISSIONRNAi@sial.com.


Deconvolution of shRNA Pools

Sigma's deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clones gives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

Go directly to:
The MISSION LentiPlex System Overview
Flow Sorting of Cells Transduced With the MISSION LentiPlex Pooled shRNA Library
Contact Us


The MISSION LentiPlex Pooled shRNA Library System Overview

The LentiPlex Pooled shRNA Libraries contain the TRC collection(s) in matrix tubes. The pooled virus is added to your cell population at a predetermined MOI. Cells are incubated then exposed to selective pressure and selected based on desired phenotype or flow sorting. After selection, cells are then isolated by colony cloning or FACS and the integrated shRNA is identified via PCR, microarray or "Next Generation" sequencing. The identity of potential "hits" can then be used to develop hypotheses regarding the biological role of the corresponding gene(s).

MISSION LentiPlex Pooled Library System Overview Image



Flow Sorting of Cells Transduced with the MISSION LentiPlex Pooled Library

Figure 1. Flow Sorting of Cells Transduced with the MISSION LentiPlex Pooled shRNA Library
J774 murine macrophage-like cells were transduced with the pooled mouse shRNA library and analyzed by flow cytometry. Transduced cells were selected in puromycin prior to FACS analysis. Both transduced and untransduced cells were stained with a mixture of FITC- and APC-conjugated primary antibodies. Cells with strong APC and weaker FITC staining were discovered and isolated as "hits" in the screen. These cells are indicated by the arrow in the upper left side of the contour plot (right). The TRC-designed pLKO.1 vector of the LentiPlex pooled constructs enables the isolation of populations using most common fluorescent reporters including FITC and GFP.

 

Contact Us

For questions about ExpressMag or other MISSION lentiviral/RNAi products, please email us at: MISSIONRNAi@sial.com.

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