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Easily Identify Hits from Pooled shRNA ScreensNeed help with deconvolution of pooled shRNA samples after screening is complete? Sigma provides a pooled shRNA deconvolution service that will help you identify TRC clones that are of significance in your screen.
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Use the form below to guide the deconvolution discussion with your Sigma® Life Science representative.
Deconvolution Sample Submission Worksheet
(628 Kb PDF)
How it works:
Pooled Screening Approach to Identify Modulators of a Pathway
Get your pooled shRNA from us, conduct your pooled screen at a lower cost and let us help you identify the genes that emerge from your screen. We will deconvolute your pooled shRNA screen with high-throughput sequencing to identify important genes.
DNA Preparation and Deconvolution Workflow
Once you provide us with cell or tumor samples post screening, we will extract genomic DNA, conduct our proprietary sequencing reaction and provide data on the presence of each TRC clone in each sample. Using our proprietary sequencing technique, we can decode the entire TRC library within a single sequencing reaction. Now we are able to serve your entire pooled shRNA screening workflow.
Why Use Next-Gen Sequencing Over Other Methods for Pooled Screen Deconvolution?
- Massive data collection without TA cloning
- Greater dynamic range
- Look for enriched or reduced shRNA
- No hybridization bias, reducing false negatives
- No cross-hybridization, reducing false positives
- Precise count of shRNA frequency
- Detects very low to high abundance shRNA
- Complete analysis without isolating single cells
- Analyze multiple pools simultaneously
- Can be conducted on custom and genome-wide pools
Example of Data Provided from Next-Gen Sequencing
