MISSION Targeted Integration shRNA Kit, Powered by CompoZr®
What is the targeted integration shRNA kit?
Some shRNA researchers have found that random lentiviral integration can sometimes interfere with non-targeted genes, causing an undesired phenotype. The MISSION Targeted Integration shRNA kit sets out to eliminate this result. The kit enables researchers to insert their shRNA into the AAVS1 locus in the human genome using our CompoZr zinc finger technology. The AAVS1 locus is a “safe harbor site” that has been validated for safe expression of a transgene1,2. This allows targeted knockdown of gene expression from a single, defined locus. Targeted insertion of shRNA into a single locus eliminates random lentiviral integration, reducing off-target effects. This kit is produced custom with your specified shRNA pre-cloned into the donor vector, making it ready-to-use with your cell line of choice.
Features and Benefits
To inquire about or order our MISSION Targeted Integration shRNA Kit, please contact your local Sigma sales representative or click below and we will put you in touch with a sales rep who will place the order.
*Custom cloning of any shRNA sequence from our vast MISSION shRNA library from the TRC or choose your own shRNA sequence
Targeted shRNA integration in K562 cells
Figure 1. The indicated shRNA-encoding sequence was delivered to K562 cells with mRNA encoding a pair of ZFN-reagents required for integration of the shRNA-expression construct into the AAVS1 site. Expression of the targeted mRNA following knockdown was measured by qRT-PCR and compared to cells expressing a non-targeting shRNA from the AAVS1 site. Bars represent the average mRNA levels from qRT-PCR samples (n=3). Error bars indicate standard deviation.
AAVS1 shRNA knockdown comparable to lenti-shRNA knockdown
Figure 2. The indicated shRNA-encoding sequence was delivered to DLD-1 or A549 cells by lentiviral transduction or by transfection with ZFNreagents required for integration of the shRNA expression construct into the AAVS1 site. Expression of the targeted mRNA following knockdown was measured by qRT-PCR and compared to cells expressing a non-targeting shRNA. Bars represent the average mRNA levels from qRT-PCR samples (n=3). Error bars indicate standard deviation. All lentiviral data are from pooled populations. AAVS1 data for the first three targets were collected from pooled populations, and data for HIF1A were collected from a clonally isolated cell line.