shRNA shRNA Gene Families

The MISSION® TRC shRNA gene family sets are collections of genes related to specific cellular/molecular functions NCBI,Gene Ontology (GO), Protein Lounge, Your Favorite Gene (YFG), and Ingenuity Systems. Sigma Bioinformatics has methodically mapped all TRC1.5 shRNA clones to each set. Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content. Additionally, any panel can be arrayed in either 96- or 384-well plate formats. All of the gene family sets can be used at the benchtop for focused target discovery and pathway analysis.

Format	Material Provided	Quality Control
Bacterial Glycerol Stock	50 µl aliquot of bacterial glycerol stocks (terrific broth, carbenicillin at 100 µg/ml and 15% glycerol)	Sequencing Verification
Purified Plasmid DNA	A minimum of 1 µg of purified plasmid DNA is provided and is aliquoted at 40 µl per well	Sequencing Verification DNA Quantitation
Lentivirus (representational, plate titer)	4 x 50 µl aliquots of VSV-G pseudotyped lentivirus, 10% representational plate titer	Sequencing Verification p24 ELISA Titer
Lentivirus (full, individual well titer)	4 x 50 µl aliquots of VSV-G pseudotyped lentivirus, fully titered to individual wells	Sequencing Verification p24 ELISA Titer

Gene Family Set	Species	Product Numbers				Product Information
		Glycerol	Plasmid DNA	Lentivirus Rep. Titer	Lentivirus Full Titer	Gene Count	Clone Count	Avg Number Clone/Gene
Apoptosis Pathway	Human	SH1911	SH1921	SH1931	inquire	440	2882	6.6
B-Cell Activation	Human	SH2911	SH2921	SH2931	inquire	99	563	5.7
Cell Adhesion Genes	Human	SH2211	SH2221	SH2231	inquire	366	2013	5.5
Cytokine and Chemokine	Human	SH0811	SH0821	SH0831	inquire	106	525	5.0
Cytokine and Chemokine Receptors	Human	SH1311	SH1321	SH1331	inquire	94	540	5.7
Cytoskeleton Genes	Human	SH2311	SH2321	SH2331	inquire	273	1534	5.6
Epigenetic Regulators	Human	SH3011	SH3021	SH3031	inquire	10	55	5.5
DNA Repair Pathway	Human	SH1811	SH1821	SH1831	inquire	116	658	5.7
Ubiquitin Hydrolases (DUBS)	Human	SH0711	SH0721	SH0731	inquire	124	612	4.9
Extracellular Matrix Genes	Human	SH2511	SH2521	SH2531	inquire	330	1750	5.3
G-Protein Coupled Receptors (GPCRs)	Human	SH0211	SH0221	SH0231	inquire	444	2761	6.2
Helicase	Human	SH2611	SH2621	SH2631	inquire	133	676	5.1
Ion Channel	Human	SH1011	SH1021	SH1031	inquire	276	1357	4.9
JAK-STAT Pathway	Human	SH2711	SH2721	SH2731	inquire	189	1128	6.0
Kinases, complete	Human	SH0111	SH0121	SH0131	inquire	513	5141	10.0
Kinases, base	Human	inquire	inquire	inquire	inquire	508	3244	6.4
Kinases, add-on	Human	inquire	inquire	inquire	inquire	401	1897	4.7
Nuclear Hormone Receptors	Human	SH1111	SH1121	SH1131	inquire	46	218	4.7
p53 Pathway	Human	SH2411	SH2421	SH2431	inquire	239	1502	6.3
Phosphatases	Human	SH0411	SH0421	SH0431	inquire	299	2369	7.9
T-Cell Activation	Human	SH2811	SH2821	SH2831	inquire	240	1265	5.3
Tumor Supressors	Human	SH0511	SH0521	SH0531	inquire	73	443	6.1
Tyrosine Kinases	Human	inquire	inquire	inquire	inquire	133	2765	20.8
Ubiquitin Ligases (E1, E2, E3)	Human	SH2111	SH2121	SH2131	inquire	203	1025	5.0
Kinases, complete	Mouse	SM0111	SM0121	SM0131	inquire	796	3932	4.9
Phosphatase	Mouse	SM0411	SM0421	SM0431	inquire	219	1086	5.0
Tyrosine Kinase	Mouse	SM2011	SM2021	SM2031	inquire	108	537	5.0

*The MISSION production and bio-informatics team constantly reviews and quality controls clones available for a gene family set. These numbers are very close to the actual number that will be shipped, but each researcher will receive a final plate map indicating the location and exact TRCN clone numbers.

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Additional resources for ion channels research.

If your gene set of interest is not available, please inquire for custom preparation at RNAi@sial.com. We can assemble custom gene sets at any time.

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For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.

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