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Life Science > Functional Genomics & RNAi > shRNA > TRC shRNA Products > shRNA Gene Families
shRNA

shRNA Gene Families

The MISSION® TRC shRNA gene family sets are collections of genes related to specific cellular/molecular functions NCBI,Gene Ontology (GO), Protein Lounge, Your Favorite Gene (YFG), and Ingenuity Systems. Sigma Bioinformatics has methodically mapped all TRC1 and
TRC1.5 (new!) shRNA clones to each set. Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content. Additionally, any panel can be arrayed in either 96- or 384-well plate formats. All of the gene family sets can be used at the benchtop for focused target discovery and pathway analysis.


Formats Available
Format Material Provided Quality Control
Bacterial Glycerol Stock 50 µl aliquot of bacterial glycerol stocks (terrific broth, carbenicillin at 100 µg/ml and 15% glycerol) Sequencing Verification
Purified Plasmid DNA An average of 2 µg per clone (range of 400 ng – 4 µg) is provided, and is aliquoted at 40 µl per well Sequencing Verification
DNA Quantitation
Lentivirus
(representational, plate titer)
4 x 50 µl aliquots of VSV-G pseudotyped lentivirus, 10% representational plate titer Sequencing Verification
p24 ELISA Titer
Lentivirus
(full, individual well titer)
4 x 50 µl aliquots of VSV-G pseudotyped lentivirus, fully titered to individual wells Sequencing Verification
p24 ELISA Titer

Mission shRNA Gene Family Sets
Gene Family Set Species Product Numbers Product Information
Glycerol Plasmid DNA Lentivirus Rep. Titer Lentivirus Full Titer Gene Count Clone Count Avg Number Clone/Gene
Apoptosis Pathway Human SH1911 SH1921 SH1931 inquire 440 2882 6.6
B-Cell Activation Human SH2911 SH2921 SH2931 inquire 99 563 5.7
Cell Adhesion Genes Human SH2211 SH2221 SH2231 inquire 366 2013 5.5
Cytokine and Chemokine Human SH0811 SH0821 SH0831 inquire 106 525 5.0
Cytokine and Chemokine Receptors Human SH1311 SH1321 SH1331 inquire 94 540 5.7
Cytoskeleton Genes Human SH2311 SH2321 SH2331 inquire 273 1534 5.6
Epigenetic Regulators Human SH3011 SH3021 SH3031 inquire 10 55 5.5
DNA Repair Pathway Human SH1811 SH1821 SH1831 inquire 116 658 5.7
Ubiquitin Hydrolases (DUBS) Human SH0711 SH0721 SH0731 inquire 124 612 4.9
Extracellular Matrix Genes Human SH2511 SH2521 SH2531 inquire 330 1750 5.3
G-Protein Coupled Receptors (GPCRs) Human SH0211 SH0221 SH0231 inquire 444 2761 6.2
Helicase Human SH2611 SH2621 SH2631 inquire 133 676 5.1
Ion Channel Human SH1011 SH1021 SH1031 inquire 276 1357 4.9
JAK-STAT Pathway Human SH2711 SH2721 SH2731 inquire 189 1128 6.0
Kinases, complete Human SH0111 SH0121 SH0131 inquire 513 5141 10.0
Kinases, base Human inquire inquire inquire inquire 508 3244 6.4
Kinases, add-on Human inquire inquire inquire inquire 401 1897 4.7
Nuclear Hormone Receptors Human SH1111 SH1121 SH1131 inquire 46 218 4.7
p53 Pathway Human SH2411 SH2421 SH2431 inquire 239 1502 6.3
Phosphatases Human SH0411 SH0421 SH0431 inquire 299 2369 7.9
T-Cell Activation Human SH2811 SH2821 SH2831 inquire 240 1265 5.3
Tumor Supressors Human SH0511 SH0521 SH0531 inquire 73 443 6.1
Tyrosine Kinases Human inquire inquire inquire inquire 133 2765 20.8
Ubiquitin Ligases (E1, E2, E3) Human SH2111 SH2121 SH2131 inquire 203 1025 5.0
Kinases, complete Mouse SM0111 SM0121 SM0131 inquire 796 3932 4.9
Phosphatase Mouse SM0411 SM0421 SM0431 inquire 219 1086 5.0
Tyrosine Kinase Mouse SM2011 SM2021 SM2031 inquire 108 537 5.0

*The MISSION production and bio-informatics team constantly reviews and quality controls clones available for a gene family set. These numbers are very close to the actual number that will be shipped, but each researcher will receive a final plate map indicating the location and exact TRCN clone numbers.

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Additional resources for ion channels research.

If your gene set of interest is not available, please inquire for custom preparation at RNAi@sial.com. We can assemble custom gene sets at any time.




Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.



MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.

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