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siRNA
MISSION® esiRNA Specifications | ||
Quality Control
- The amplified region of the cDNA with the highest possible number of highly effective siRNA is selected using DEQOR primer design program.
- PCR product of transcriptome library tested via gel electrophoresis and identity confirmed by DNA sequencing.
- Purified RNase III digested product (esiRNA) validated via gel electrophoresis.
- esiRNA concentration determined by measuring OD260 or fluorescence intensity.
Purification
- RNase III digested products are purified via column purification with Q-Sepharose, isopropanol precipitation and ethanol wash.
Format
- All esiRNA are delivered in solution (resuspended in nuclease-free TE buffer) at the concentrations listed below, as measured by OD260 or fluorescence intensity.
- esiRNA offering: 16,432 Human gene targets with 16,744 esiRNAs
14,061 Mouse gene targets with 14,068 esiRNAs
Available MISSION esiRNA Products
| Individual esiRNAs | Plates MISSION esiRNAs | esiRNA Genome Scale Libraries | esiFLEX esiRNAs | |
| Quantities Available | 20 ug (2 nmol) 50 ug (5 nmol) |
20 ug (2 nmol) 50 ug (5 nmol) |
1 ug (0.1 nmol) 2.5 ug (0.25 nmol) |
1 ug (0.1 nmol) 2.5 ug (0.25 nmol) |
| Gene target sources available | Human Mouse |
Human Mouse |
Human Mouse |
Human Mouse |
| Packaging | 2 ml tubes | 96-well plate (customizable) | 96- or 384-well plate | 96- or 384-well plate (customizable) |
| Concentration | 200 ng/uL | 200 ng/uL | 50 ng/uL | 50 ng/uL |
| esiOPEN | esiSEC | |
| Quantities available | 20 ug (2 nmol) 50 ug (5 nmol) |
20 ug (2 nmol) 50 ug (5 nmol) |
| Gene target sources available | All species | Human Mouse |
| Packaging | 2 ml tubes | 2 ml tubes |
| Concentration | 200 ng/uL | 200 ng/uL |
- The standard plate format for MISSION esiRNA libraries is in 384-well plates, with all positions occupied.
Shipping, Storage, and Stability
- Shipped in solution on dry ice, in sealed plates.
- esiRNA stored at –20 °C have demonstrated to be stable for 2 years. No decrease in stability after 12 freeze/thaw cycles.
Certificate of Analysis/Technical Data Sheet
esiRNA will be delivered with an esiRNA Technical Data Sheet, which includes:
- Plate number and position
- esiRNA ID number
- Gene ID (Genbank) or Gene ID (Ensembl)
- Gene name
- Organism
- OD (units)
- Amount (μg)
- Concentration (ng/μL)
- Lot number
- Target sequence
- Additional data are provided for purified RNase III digested product (esiRNA) upon request.
- Custom esiRNA libraries and genome scale esiRNA libraries will be delivered with a CD containing complete plating information.