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Life Science > Functional Genomics & RNAi > siRNA > siRNA/Nano Scale
siRNA

MISSION® Predesigned siRNA

Mission siRNA

Your Favorite Gene Search Box
Your Favorite Gene Search
We've made ordering MISSION® siRNA for single gene targets easy through Sigma's state-of-the-art web interface, Your Favorite Gene.

New Predesigned MISSION siRNAs available through YFG!

On this page:
MISSION siRNA
Benefits
Validated Human siRNA
siRNA pools
Nano Scale siRNA
References
MISSION siRNA Libraries

New Predesigned MISSION siRNAs are now available based on the latest version of NCBI RefSeq. All previous Predesigned MISSION siRNAs will still be contained in YFG, but their rankings may have changed based on updated sequence information. The new siRNA sequences will have the designation 02 within the siRNA ID. For more information, contact RNAi@sial.com.

Sigma-Aldrich® has entered an exclusive partnership with Rosetta Inpharmatics, a leader in advanced siRNA research, to use the proprietary Rosetta siRNA Design Algorithm to design its MISSION line of siRNA. The Rosetta siRNA Design Algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the all-important siRNA seed region to predict the most effective and specific siRNA sequences for your target gene of interest. Additionally, the Rosetta siRNA Design Algorithm was developed utilizing data from over three years of gene-silencing experiments, ensuring that the algorithm’s in silico rules are guided and bolstered by real-world empirical evidence.

Sigma-Aldrich guarantees that two out of three siRNA duplexes per target gene will achieve knockdown efficiencies of greater than or equal to 75%.

Benefits

Two Scientists
  • Best-in-class, guaranteed gene silencing
  • Efficient knockdown of low abundance messages
  • siRNAs targeting every gene in the current human, mouse, and rat genomes
  • Simplify transfection optimization with 11 MISSION Positive Control siRNA
  • Over 700 MISSION predesigned siRNA have been functionally validated
  • Individual MISSION predesigned siRNA or Pre-arrayed siRNA Libraries

Validated Human MISSION siRNA

Many common gene targets, including VEGFC, PLK4, and JAK1, have been validated for ≥75% mRNA knockdown. Validated MISSION siRNA are suitable for transfection optimization and as positive controls.

Validation Data

Knockdown Graph
HeLa cells transfected at a concentration of 30 nM siRNA. Percentage remaining gene expression levels measured via quantitative real-time PCR 48 hours after transfection (relative to mock). Data represents the mean of four biological replicates.

siRNA pools

siRNA Pool

MISSION predesigned or custom siRNA(including modifications) are available in pooled format.

  • Yield: 2OD/10nmol per sequence*
  • Supplied as 5nmol per duplex in pool format, with 5nmol of the individual duplex also provided
  • Up to 4 duplexes/pool*
  • Purification: Desalt and HPLC*
  • Packaging: 1 tube containing pooled material, and individual tubes for each duplex ordered
  • Turn around Time: 7 business days

*Additional customization is available
Please contact your local sales representative or email us at acctreps-hou@sial.com for additional information or to place your order.

siRNA Kinase Nano Scale

A convenient, cost-effective, and small scale siRNA screening option. Our MISSION siRNA Nano Scale targets are manufactured using an siRNA Design Algorithm that significantly reduces off-target effects and ensures effective target gene knockdown.

Ordering Information

You can now order predesigned MISSION siRNAs targeting kinase genes at the 0.25 nmol scale. Easily find your MISSION siRNA Kinase Nano Scale target by inserting your gene of interest into the search box above.



Selected Citations—MISSION Predesigned siRNA and Pre-arrayed siRNA Libraries

Gilot, D. et al. RNAi-Based Screening Identifies Kinases Interfering with Dioxin-Mediated Up-Regulation of CYP1A1 Activity. PLoS ONE 2011, 6(3): e18261

Meng, W. et al. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell 2008 Nov 28, 135(5), 948-59.

Matsubara, T. et al. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J. Biol. Chem. Oct 2008, 283(43), 29119-25. Epub 2008 Aug 14.

Zhou, H. et al. Genome-Scale RNAi Screen for Host Factors Required for HIV Replication. Cell Host Microbe 2008 Oct 29. [Epub ahead of print].

Marine, S. et al. Activity profile-based siRNA screen to explore the functional genomics of Alzheimer’s disease. BioTechniques Dec 2007, 43, Supplement, Large-Scale Genomic Analysis: S22–S27.

Espeseth, A. S. et al. Genome wide analysis of ubiquitin ligases in APP processing identifies a novel regulator of BACE1 mRNA levels. Mol. Cell. Neurosci. Nov 2006, 33(3), 227–35.

Bartz, S. R. et al. Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptions. Mol. Cell. Biol. Dec 2006, 26(24), 9377–86.

Majercak, J. et al. LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer’s disease. Proc. Natl. Acad. Sci. U.S.A. Nov 2006, 103(47), 17967–72.


Pre-arrayed MISSION siRNA Libraries Ordering Information

MISSION siRNA Human Druggable Genome

MISSION siRNA Rat Druggable Genome

MISSION siRNA Mouse Kinase Library


Contact Us

For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.

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