Sigma-Aldrich® has entered an exclusive partnership with Rosetta Inpharmatics, a leader in advanced siRNA research, to use the proprietary Rosetta siRNA Design Algorithm to design its MISSION line of siRNA. The Rosetta siRNA Design Algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the all-important siRNA seed region to predict the most effective and specific siRNA sequences for your target gene of interest. Additionally, the Rosetta siRNA Design Algorithm was developed utilizing data from over three years of gene-silencing experiments, ensuring that the algorithm’s in silico rules are guided and bolstered by real-world empirical evidence.
Sigma-Aldrich guarantees that two out of three siRNA duplexes per target gene will achieve knockdown efficiencies of greater than or equal to 75%.
Benefits of predesigned MISSION siRNA, powered by the Rosetta siRNA Design Algorithm:
- Best-in-class, guaranteed gene silencing
- Efficient knockdown of low abundance messages
- siRNAs targeting every gene in the current human, mouse, and rat genomes
- Simplify transfection optimization with 11 MISSION Positive Control siRNA
- Over 700 MISSION predesigned siRNA have been functionally validated
- Individual MISSION predesigned siRNA or Pre-arrayed siRNA Libraries
MISSION Predesigned siRNA provide best-in-class gene silencing
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HeLa cells transfected at a concentration of 30 nM siRNA. Percentage remaining gene expression levels measured via quantitative real-time PCR 48 hours after transfection (relative to mock). Data represents the mean of four biological replicates.
Lower Score = Increased Gene Silencing |
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Selected Citations—MISSION Predesigned siRNA and Pre-arrayed siRNA Libraries
Meng, W. et al. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell 2008 Nov 28, 135(5), 948-59.
Matsubara, T. et al. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J. Biol. Chem. Oct 2008, 283(43), 29119-25. Epub 2008 Aug 14.
Zhou, H. et al. Genome-Scale RNAi Screen for Host Factors Required for HIV Replication. Cell Host Microbe 2008 Oct 29. [Epub ahead of print].
Marine, S. et al. Activity profile-based siRNA screen to explore the functional genomics of Alzheimer’s disease. BioTechniques Dec 2007, 43, Supplement, Large-Scale Genomic Analysis: S22–S27.
Espeseth, A. S. et al. Genome wide analysis of ubiquitin ligases in APP processing identifies a novel regulator of BACE1 mRNA levels. Mol. Cell. Neurosci. Nov 2006, 33(3), 227–35.
Bartz, S. R. et al. Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptions. Mol. Cell. Biol. Dec 2006, 26(24), 9377–86.
Majercak, J. et al.LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer’s disease. Proc. Natl. Acad. Sci. U.S.A. Nov 2006, 103(47) 17967–72.
Pre-arrayed MISSION siRNA Libraries Ordering Information
MISSION siRNA Human Druggable Genome
MISSION siRNA Rat Druggable Genome
MISSION siRNA Mouse Kinase Library
Contact Us
For questions about the library, pricing and quotes or other concerns, please e-mail us at: RNAi@sial.com.
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Label License.
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