siRNA

MISSION® Predesigned siRNA

Mission siRNA


 

We've made ordering MISSION® siRNA for single gene targets easy through Sigma's
state-of-the-art web interface, Your Favorite Gene.

New Predesigned MISSION siRNAs available through YFG!

On this page:
MISSION siRNA
Benefits
Validated Human siRNA
siRNA pools
Nano Scale siRNA
References
MISSION siRNA Libraries

New Predesigned MISSION siRNAs are now available based on the latest version of NCBI RefSeq. All previous Predesigned MISSION siRNAs will still be contained in YFG, but their rankings may have changed based on updated sequence information. The new siRNA sequences will have the designation 02 within the siRNA ID. For more information, contact MISSIONRNAi@sial.com.

Sigma-Aldrich has entered an exclusive partnership with Rosetta Inpharmatics, a leader in advanced siRNA research, to use the proprietary Rosetta siRNA Design Algorithm to design its MISSION line of siRNA. The Rosetta siRNA Design Algorithm utilizes Position-Specific Scoring Matrices (PSSM) and knowledge of the all-important siRNA seed region to predict the most effective and specific siRNA sequences for your target gene of interest. Additionally, the Rosetta siRNA Design Algorithm was developed utilizing data from over three years of gene-silencing experiments, ensuring that the algorithm’s in silico rules are guided and bolstered by real-world empirical evidence.

Sigma-Aldrich guarantees that two out of three siRNA duplexes per target gene will achieve knockdown efficiencies of greater than or equal to 75%.

Benefits

Two Scientists
  • Best-in-class, guaranteed gene silencing
  • Efficient knockdown of low abundance messages
  • siRNAs targeting every gene in the current human, mouse, and rat genomes
  • Simplify transfection optimization with 11 MISSION Positive Control siRNA
  • Over 700 MISSION predesigned siRNA have been functionally validated
  • Individual MISSION predesigned siRNA or Pre-arrayed siRNA Libraries

Validated Human MISSION siRNA

Many common gene targets, including VEGFC, PLK4, and JAK1, have been validated for ≥75% mRNA knockdown. Validated MISSION siRNA are suitable for transfection optimization and as positive controls.

Validation Data

Knockdown Graph
HeLa cells transfected at a concentration of 30 nM siRNA. Percentage remaining gene expression levels measured via quantitative real-time PCR 48 hours after transfection (relative to mock). Data represents the mean of four biological replicates.

siRNA pools

siRNA Pool

MISSION predesigned or custom siRNA(including modifications) are available in pooled format.

  • Yield: 2OD/10nmol per sequence*
  • Supplied as 5nmol per duplex in pool format, with 5nmol of the individual duplex also provided
  • Up to 4 duplexes/pool*
  • Purification: Desalt and HPLC*
  • Packaging: 1 tube containing pooled material, and individual tubes for each duplex ordered
  • Turn around Time: 7 business days

*Additional customization is available.
Note: Pools available in North America only at this time.
Please contact your local sales representative or email us at acctreps-hou@sial.com for additional information or to place your order.

Nano Scale siRNA

A convenient, cost-effective, and small scale siRNA screening option. Our MISSION siRNA Nano Scale targets are manufactured using an siRNA Design Algorithm that significantly reduces off-target effects and ensures effective target gene knockdown.

  • Available as individual siRNA targets or as a gene family set
  • siRNA targeting human kinases or phosphatases at the 0.25 nmol scale
  • 21mer siRNA duplexes with dTdT overhangs

MISSION siRNA Human Kinase Panel (Cat. No. SI02100)

  • Targets 719 kinase genes, encompassing 2,490 siRNA
  • Format: 96 well plates with 80 duplexes per plate; delivered dry with a final quantity of 0.25 nmol per duplex
  • First and last columns of the plate have been left empty for the addition of controls
  • Electronic file supplied including plate maps, target gene list and full sequence information

MISSION siRNA Human Phosphatase Panel (Cat. No. SI03200)

  • Targets 303 phosphatase genes, encompassing 1,131 siRNA
  • Format: 96 well plates; delivered dry with a final quantity of 0.25 nmol per duplex
  • First and last columns of the plate have been left empty for the addition of controls
  • Electronic file supplied including plate maps, target gene list and full sequence information

Ordering Information

You can now order predesigned MISSION siRNAs targeting kinase or phosphatase genes at the 0.25 nmol scale. Search alphabetically by gene symbol to easily find your individual MISSION siRNA Kinase or Phosphatase Nano Scale target.

Selected Citations—MISSION Predesigned siRNA and Pre-arrayed siRNA Libraries

Yang, X. et al. Gene Silencing Activity of siRNA Molecules Containing Phosphorodithioate Substitiutions. ACS Chem. Biol. 2012, 7 (7): 1214-1220. 22512638

Salma, J., & McDermott, J. Suppression of a MEF2-KLF6 Survival Pathway by PKA Signaling Promotes Apoptosis in Embryonic Hippocampal Neurons. Journal of Neuroscience, 32(8), 2790-2803 (2012).

Gilot, D. et al. RNAi-Based Screening Identifies Kinases Interfering with Dioxin-Mediated Up-Regulation of CYP1A1 Activity. PLoS ONE 2011, 6(3): e18261

Raab, M., et al. Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells. Nat Commun. 2011 Jul 19;2:395. doi: 10.1038/ncomms1395.

Chia, K., et al. A Feedback Loop between Androgen Receptor and ERK Signaling in Estrogen Receptor-Negative Breast Cancer. Neoplasia. 2011 Feb, 13(2): 154–166. PMC3033594

Ramachandran, V., et al. The ADMR receptor mediates the effects of adrenomedullin on pancreatic cancer cells and on cells of the tumor microenvironment. PLoS One. 2009 Oct 22;4(10):e7502.

Sanatra, M.K., et al. F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arrest after DNA damage. Nature. 2009 Jun 4;459(7247):722-5. Epub 2009 May 3.

Meng, W. et al. Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts. Cell 2008 Nov 28, 135(5), 948-59.

Matsubara, T. et al. BMP2 regulates Osterix through Msx2 and Runx2 during osteoblast differentiation. J. Biol. Chem. Oct 2008, 283(43), 29119-25. Epub 2008 Aug 14.

Zhou, H. et al. Genome-Scale RNAi Screen for Host Factors Required for HIV Replication. Cell Host Microbe 2008 Oct 29. [Epub ahead of print].

Marine, S. et al. Activity profile-based siRNA screen to explore the functional genomics of Alzheimer’s disease. BioTechniques Dec 2007, 43, Supplement, Large-Scale Genomic Analysis: S22–S27.

Espeseth, A. S. et al. Genome wide analysis of ubiquitin ligases in APP processing identifies a novel regulator of BACE1 mRNA levels. Mol. Cell. Neurosci. Nov 2006, 33(3), 227–35.

Bartz, S. R. et al. Small interfering RNA screens reveal enhanced cisplatin cytotoxicity in tumor cells having both BRCA network and TP53 disruptions. Mol. Cell. Biol. Dec 2006, 26(24), 9377–86.

Majercak, J. et al. LRRTM3 promotes processing of amyloid-precursor protein by BACE1 and is a positional candidate gene for late-onset Alzheimer’s disease. Proc. Natl. Acad. Sci. U.S.A. Nov 2006, 103(47), 17967–72.

Pre-arrayed MISSION siRNA Libraries Ordering Information

MISSION siRNA Human Druggable Genome

MISSION siRNA Rat Druggable Genome

MISSION siRNA Mouse Kinase Library

 

Contact Us

For questions about the library, pricing and quotes or other concerns, please e-mail us at: MISSIONRNAi@sial.com.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC Label License.

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