N-TER

siRNA Delivery to Hard-to-Transfect Cell Types

Traditional lipid-based siRNA transfection reagents exhibit a number of drawbacks, including a limited ability to transfect into a variety of cell types, such as primary, neuronal, differentiated, and non-dividing cells.

Sigma’s N-TER Nanoparticle siRNA Transfection System is based on a peptide transfection reagent specifically designed to bypass these limitations and allow for efficient delivery of siRNAs into these historically recalcitrant eukaryotic cell types.

Benefits of the N-TER Nanoparticle siRNA Transfection System:

• Superior transfection of historically difficult-to-transfect cells, including primary, neuronal, differentiated and non-dividing cells
• Quick delivery of siRNA into cells, with reduced cytotoxicity as compared to lipid-based reagents
• Stable N-TER/siRNA nanoparticles can be stored at -20 °C for up to 1 year
• Simple protocol easily adapted for high-throughput and reverse transfection applications

N-TER Quickly Delivers siRNA into Cells
	Click here to view a larger image.
	Click here for an overview of the simple protocol for N-TER/siRNA transfection.

Selected References

1. Morris, M. C., et al. A new peptide vector for the efficient delivery of oligonucleotides into mammalian cells. Nucleic Acids Res. 1997, 25, 2730-2736.
2. Simeoni, F., et al. Insight into the mechanism of the peptide-based gene delivery system MPG: Implications for delivery of siRNA into mammalian cells. Nucleic Acids Res. 2003, 31, 2717-2724.
3. Morris, K. V., et al. Small interfering RNA induced transcriptional gene silencing in human cells. Science 2004, 305, 1289-1291.
4. Deshayes, S., et al. On mechanism of non-endosomial peptide-mediated cellular delivery of nucleic acids. Biochem. Biophys. Acta. 2004, 1667(2), 141-147.
5. Langlois, M. A., et al. Cytoplasmic and nuclear retained DMPK mRNAs are targets for RNA interference in myotonic dystrophy cells. J. Biol. Chem. 2005, 280(17), 16949-16954.

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