siRNA

N-TER™

N-TER Nanoparticle siRNA Transfection System

siRNA Delivery to Hard-to-Transfect Cell Types

Traditional lipid-based siRNA transfection reagents exhibit a number of drawbacks, including a limited ability to transfect into a variety of cell types, such as primary, neuronal, differentiated, and non-dividing cells.

Sigma’s N-TER Nanoparticle siRNA Transfection System is based on a peptide transfection reagent specifically designed to bypass these limitations and allow for efficient delivery of siRNAs into these historically recalcitrant eukaryotic cell types.

Benefits of the N-TER Nanoparticle siRNA Transfection System:

  • Superior transfection of historically difficult-to-transfect cells, including primary, neuronal, differentiated and non-dividing cells
  • Quick delivery of siRNA into cells, with reduced cytotoxicity as compared to lipid-based reagents
  • Stable N-TER/siRNA nanoparticles can be stored at -20 °C for up to 1 year
  • Simple protocol easily adapted for high-throughput and reverse transfection applications

 

Product No. Description Add to Cart
N2913-120UL N-TER Nanoparticle siRNA Transfection System
N2913-400UL N-TER Nanoparticle siRNA Transfection System
N2913-1ML N-TER Nanoparticle siRNA Transfection System

 

N-TER Quickly Delivers siRNA into Cells
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Table 1
Cell lines that N-TER has been successfully used to transfect siRNA

Cell Type Source Tissue
3T3-L1, differentiated adipocyte mouse embryonic fibroblast cell line
A2780 human ovarian carcinoma cell line
A549 human lung carcinoma cell line
ASPC-1 human pancreatic carcinoma cell line
Astrocyte human neuronal primary cell
Astrocytoma human neuronal astrocytoma cell line
BSMC human bronchial smooth muscle primary cell
C2C12, differentiated myocyte mouse myoblastoma line
HeLa human cervical adenocarcinoma cell line
HepG2 human hepatocarcinoma cell line
HT-29 human colorectal adenocarcinoma cell line
HUVEC human umbilical vein endothelial primary cell
MCF7 human breast adenocarcinoma cell line
MDA-MB-231 human breast adenocarcinoma cell line
NHEK-AD human adult keratinacyte primary cell
Raw264.7 mouse macrophage cell line
SW620 human colorectal adenocarcinoma cell line
U-87 MG human brain glioblastoma cell line

 

N-TER Cell Types and Protocol Click here for an overview of the simple protocol for N-TER/siRNA transfection.



Selected References

  1. Morris, M. C., et al. A new peptide vector for the efficient delivery of oligonucleotides into mammalian cells. Nucleic Acids Res. 1997, 25, 2730-2736.
  2. Simeoni, F., et al. Insight into the mechanism of the peptide-based gene delivery system MPG: Implications for delivery of siRNA into mammalian cells. Nucleic Acids Res. 2003, 31, 2717-2724.
  3. Morris, K. V., et al. Small interfering RNA induced transcriptional gene silencing in human cells. Science 2004, 305, 1289-1291.
  4. Deshayes, S., et al. On mechanism of non-endosomial peptide-mediated cellular delivery of nucleic acids. Biochem. Biophys. Acta. 2004, 1667(2), 141-147.
  5. Langlois, M. A., et al. Cytoplasmic and nuclear retained DMPK mRNAs are targets for RNA interference in myotonic dystrophy cells. J. Biol. Chem. 2005, 280(17), 16949-16954.

 

 

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