Predesigned Guarantee

Upon purchase of three MISSION Predesigned siRNA targeting the same gene, at least two of the siRNA will reduce target mRNA levels by 75% or greater, in cultured cells, when transfected at 30 nM or greater. If two of the siRNA do not knockdown the target gene by 75%, Sigma® will provide three additional siRNA for that gene target free of charge. In the case that there are no more siRNA for that gene target or all siRNA fail to knock down the target gene by 75% or more, Sigma will refund the price of the target set. Receipt of appropriate supporting data for transfection efficiency is required for the guarantee. Appropriate supporting data for transfection efficiency would include quantitative PCR based silencing data, comparing target mRNA levels of a MISSION Positive Control siRNA (GAPDH, MAPK1, TP53, etc.), transfected at 30 nM or greater, to an appropriate negative control (such as mock transfection or a scrambled sequence siRNA), demonstrating knockdown of the target mRNA greater than or equal to 75%. Due to the variability of antibodies and protein half-lives, we are unable to accept protein based detection methods.